SMAD2 ELISA Kit

Produktnummer ABIN7672241

Ratte SMAD2 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Ratte SMAD2.

Kurzübersicht für SMAD2 ELISA Kit (ABIN7672241)

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.625 ng/mL - 40 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Güteklasse: QuickTest

Produktdetails SMAD2 ELISA Kit

Untere Nachweisgrenze: 0.625 ng/mL

Verwendungszweck: Rat Smad2 (Mothers against decapentaplegic homolog 2) QuickTest ELISA Kit

Marke: QuickTest

Analytische Methode: Quantitative

Sensitivität: 0.375 ng/mL

Bestandteile:

• ELISA Microplate (Dismountable)
• Lyophilized Standard
• Capture/Detection Antibody
• HRP-Streptavidin (orange)
• TMB Substrate
• Sample Dilution Buffer (blue)
• Stop Solution
• Wash Buffer (25x)
• Plate Sealer
• Product Description

Benötigtes Material:

• Microplate reader (wavelength: 450nm)
• 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
• Automated plate washer or multi-channel pipette/5 mL pipettor (for manual washing purpose)
• Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips (Calibration is required before use.)
• Sterile tubes and sample tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Anwendungsinformationen

Kommentare:

Traditional sandwich ELISA assay usually takes 3-4 hours. Most detection reagents in the market are concentrated and can't be distinguished from the colour. Dilution is required before use. We developed QuickTest ELISA kits to decrease customers’ operational errors, improve user's experience and get accurate assay result more efficiently. Compared with regular ELISA kits, QuickTest ELISA kits have more advantages in the same linearity, stability, recovery and storage conditions.

Probenmenge: 50 µL

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody pre-coated on the QuickTest plate. Add the Cap/Det Ab working solution into each well, then add the standards and pilot samples into relevant wells. Mix thoroughly and then incubate. If the sample contains detection target, a capture antibody-detection target-biotin-detection antibody complex was formed. After washing off unbound conjugates, HRP-Streptavidin was added to incubate. Repeat the washing process and then TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm with a microplate reader. The concentration of the detection target in the sample was calculated by plotting a standard curve. The concentration of the target substance is proportional to the OD450 value.

Testdurchführung:

1. Take out the required strips, add 50 µL Cap/Det Ab into each well, then add 50 µL Standard or Sample into relevant well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure complete mixing then statically incubate for 60 minutes at 37 °C.
2. Wash the plate twice without immersion.
3. Add 100 µL HRP-Streptavidin (orange) into each well, seal the plate and statically incubate for 30 minutes at 37 °C.
4. Wash the plate five times without immersion.
5. Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C (Accurate TMB visualization control is required.).
6. Add 50 µL stop solution. Read at 450nm immediately and calculate.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 2-8 °C for 12 months

Haltbarkeit: 12 months

Details zu SMAD2

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Andere Bezeichnung: Smad2

Hintergrund: Mothers against decapentaplegic homolog 2|MAD homolog 2|Mothers against DPP homolog 2|JV18-1|Mad-related protein 2|hMAD-2|SMAD family member 2|SMAD 2, Smad2, hSMAD2|SMAD2|MADH2|MADR2

UniProt: O70436

Pathways: Zellzyklus, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus