Adipsin ELISA Kit

Produktnummer ABIN7671210

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human Adipsin.

Kurzübersicht für Adipsin ELISA Kit (ABIN7671210)

Target: Adipsin (CFD) (Complement Factor D (CFD))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.313 ng/mL - 20 ng/mL

Applikation: ELISA

Proben: Biological Fluids, Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Güteklasse: Quick

Produktdetails Adipsin ELISA Kit

Untere Nachweisgrenze: 0.313 ng/mL

Verwendungszweck: Human CFD (Complement factor D) QuickTest ELISA Kit

Analytische Methode: Quantitative

Spezifität: Specifically binds with CFD , no obvious cross reaction with other analogues.

Sensitivität: 0.188 ng/mL

Bestandteile:

• ELISA Microplate(Dismountable)
• Lyophilized Standard
• Cap/Det Ab (Ready to use
• blue)
• TMB Substrate
• Sample Dilution Buffer
• Stop Solution
• Plate Sealer
• Product Description

Benötigtes Material:

• Microplate reader (wavelength: 450nm)
• Microorifice oscillator
• or shake bed 37 °C incubator (When using a water bath incubator
• ensure the internal air temperature is 35-38 °C, When using the cell CO2 incubator
• use a sealed bag to isolate the microplate)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Sandwich ELISA, Double Antibody

Probenmenge: 50 μL

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: Step 1: Take out the required plate wells, add 50 µL Cap/Det Ab into each well, then add 50 µL Standard or Sample into individual well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Seal the plate and incubate on a microplate oscillator or shaker at 200 rpm (2-8 °C) for 1 hour, ensuring no spillage from any well. Washing: Wash the plate twice without immersion. Step 2: Add 100 µL HRP-Streptavidin (orange) into each well, Seal the plate and incubate on a microplate oscillator or shaker at 200 rpm and room temperature (25 °C ± 3 °C) for 30 hours, ensuring no spillage from any well. Washing: Wash the plate five times without immersion. Step 3: Add 90 µL TMB substrate solution, seal the plate and static incubate for 15 minutes at room temperature (25 °C ± 3 °C) in the dark. (Accurate TMB visualization control is required.) Step 4: Add 50 µL stop solution. Read at 450nm immediately and calculate.

Testdurchführung:

1. Take out the required strips, add 50 µL Cap/Det Ab into each well, then add 50 µL Standard or Sample into relevant well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure complete mixing then statically incubate for 60 minutes at 37 °C.
2. Wash the plate twice without immersion.
3. Add 100 µL HRP-Streptavidin (orange) into each well, seal the plate and statically incubate for 30 minutes at 37 °C.
4. Wash the plate five times without immersion.
5. Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C (Accurate TMB visualization control is required.).
6. Add 50 µL stop solution. Read at 450nm immediately and calculate.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid direct light exposure to antibody solutions

Lagerung: 4 °C

Informationen zur Lagerung: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Haltbarkeit: 12 months

Details zu Adipsin

Target: Adipsin (CFD) (Complement Factor D (CFD))

Andere Bezeichnung: CFD

Hintergrund: Complement factor D, Adipsin, C3 convertase activator, Properdin factor D, CFD, DF, PFD

UniProt: P00746

Pathways: Komplementsystem