ACE2 ELISA Kit

Produktnummer ABIN6952471

Dieses Human ACE2 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human ACE2 zu quantifizieren.

Kurzübersicht für ACE2 ELISA Kit (ABIN6952471)

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.025 ng/mL - 20 ng/mL

Applikation: ELISA

Proben: Cell Culture Cells, Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Produktdetails ACE2 ELISA Kit

Untere Nachweisgrenze: 0.025 ng/mL

Verwendungszweck: Human ACE-2 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects Human ACE-2. Other species not determined.

Produktmerkmale: Human ACE-2 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water/li>
• Precision pipettes to deliver 2 μL to 1 μL volumes/li>
• Adjustable 1-25 μL pipettes for reagent preparation/li>
• 100 μL and 1 liter graduated cylinders/li>
• Tubes to prepare standard and sample dilutions/li>
• Absorbent paper/li>
• Microplate reader capable of measuring absorbance at 450nm/li>
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Aufbereitung der Proben:

Recommended Dilution (serum & plasma):8 fold

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Details zu ACE2

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Andere Bezeichnung: ACE-2

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour