ATG10 ELISA Kit

Produktnummer ABIN6951722

Produktdetails ATG10 ELISA Kit

Target: ATG10 (Autophagy Related 10 (ATG10))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human ATG10 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu ATG10

Target: ATG10 (Autophagy Related 10 (ATG10))

Andere Bezeichnung: ATG10 (ATG10 Produkte)

Synonyme: APG10 ELISA Kit, APG10L ELISA Kit, pp12616 ELISA Kit, Atg10 ELISA Kit, ATG10 ELISA Kit, MGC123341 ELISA Kit, im:7153729 ELISA Kit, zgc:123341 ELISA Kit, ATATG10 ELISA Kit, autophagy 10 ELISA Kit, DDBDRAFT_0190076 ELISA Kit, DDBDRAFT_0252608 ELISA Kit, DDB_0190076 ELISA Kit, DDB_0252608 ELISA Kit, 5330424L23Rik ELISA Kit, 5430428K15Rik ELISA Kit, AI852123 ELISA Kit, Agp10 ELISA Kit, Apg10l ELISA Kit, Apg10p ELISA Kit, Atg10l ELISA Kit, autophagy related 10 ELISA Kit, autophagy-related 10 ELISA Kit, AuTophaGy (yeast Atg homolog) ELISA Kit, ATG10 autophagy related 10 homolog (S. cerevisiae) ELISA Kit, autophagocytosis-associated family protein ELISA Kit, E2-like conjugating enzyme ELISA Kit, hypothetical protein ELISA Kit, autophagy protein 10 ELISA Kit, autophagy-related protein 10 ELISA Kit, ATG10 ELISA Kit, Atg10 ELISA Kit, atg-10 ELISA Kit, atg10 ELISA Kit, CAALFM_C302930WA ELISA Kit, APG10 ELISA Kit

Gen-ID: 83734

UniProt: Q9H0Y0

Pathways: ER-Nucleus Signaling, Autophagie