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B-Cell Linker ELISA Kit

BLNK Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951540
  • Target Alle B-Cell Linker (BLNK) ELISA Kits anzeigen
    B-Cell Linker (BLNK)
    Reaktivität
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human BLNK ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product BLNK ELISA Kit
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Haltbarkeit
    6 months
  • Target Alle B-Cell Linker (BLNK) ELISA Kits anzeigen
    B-Cell Linker (BLNK)
    Andere Bezeichnung
    BLNK (BLNK Produkte)
    Synonyme
    BLNK ELISA Kit, blnk ELISA Kit, MGC147045 ELISA Kit, BASH ELISA Kit, Bca ELISA Kit, Ly-57 ELISA Kit, Ly57 ELISA Kit, Lyw-57 ELISA Kit, SLP-65 ELISA Kit, AGM4 ELISA Kit, BLNK-S ELISA Kit, LY57 ELISA Kit, SLP65 ELISA Kit, bca ELISA Kit, B-cell linker ELISA Kit, B cell linker ELISA Kit, BLNK ELISA Kit, blnk ELISA Kit, Blnk ELISA Kit
    Gen-ID
    29760
    UniProt
    Q8WV28
    Pathways
    BCR Signaling
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