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Beclin 1 ELISA Kit

BECN1 Reaktivität: Human, Ratte, Maus pSer234 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Produktnummer ABIN6951538
  • Target Alle Beclin 1 (BECN1) ELISA Kits anzeigen
    Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))
    Bindungsspezifität
    pSer234
    Reaktivität
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Ratte, Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human/Mouse/Rat Phospho-Beclin-1 (S234) ELISA Kit. This assay semi-quantitatively measures phosphorylated Beclin-1 (Ser234) in lysate samples.
    Proben
    Cell Lysate, Tissue Lysate
    Analytische Methode
    Semi-Quantitative
    Spezifität
    The antibody pair provided in this kit recognizes human, mouse, and rat Beclin-1 phosphorylated at site Serine-234.
    Produktmerkmale
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Benötigtes Material
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Haltbarkeit
    6 months
  • Target Alle Beclin 1 (BECN1) ELISA Kits anzeigen
    Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))
    Andere Bezeichnung
    Beclin-1 (BECN1 Produkte)
    Synonyme
    beclin-1 ELISA Kit, atg6 ELISA Kit, vps30 ELISA Kit, beclin1 ELISA Kit, Beclin-1 ELISA Kit, becn1 ELISA Kit, zgc:73097 ELISA Kit, ATG6 ELISA Kit, VPS30 ELISA Kit, 4921513J16Rik ELISA Kit, 5430417M23Rik ELISA Kit, Atg6 ELISA Kit, beclin 1 ELISA Kit, beclin 1, autophagy related ELISA Kit, beclin 1 protein ELISA Kit, beclin-1 ELISA Kit, beclin family protein ELISA Kit, beclin 1, autophagy related L homeolog ELISA Kit, BECN1 ELISA Kit, becn1 ELISA Kit, atg6 ELISA Kit, CpipJ_CPIJ011087 ELISA Kit, PTRG_07825 ELISA Kit, SJAG_01876 ELISA Kit, Becn1 ELISA Kit, becn1.L ELISA Kit
    Gen-ID
    8678
    Pathways
    Autophagie
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