ATG4B ELISA Kit
                                    Human ATG4B ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human ATG4B.
            
                        
         
        
                        
                Produktnummer ABIN6951529
                            
                                                
                                
    
                
                                    
                    
                            
            
         
                
            
    Kurzübersicht für ATG4B ELISA Kit (ABIN6951529)
                        
                Target
            
                                                Alle ATG4B Produkte
                                        
                ATG4B
                                    (Autophagy related 4B Cysteine Peptidase (ATG4B))
                            
                                    
                Reaktivität
            
                        
                Human
            
                                    
                Nachweismethode
            
                        
                Colorimetric
            
                                    
                Methodentyp
            
                        
                Sandwich ELISA
            
                                    
                Applikation
            
                        
                ELISA
            
                                        
                Proben
            
                        
                Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
            
                                         
                
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                                            Verwendungszweck
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                        Human ATG4B ELISA Kit.
                                            
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                                            Analytische Methode
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                        Quantitative
                                            
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                                            Produktmerkmale
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                        - Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
 
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                                            Bestandteile
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                        - Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
 
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                                            Benötigtes Material
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                        - Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
 
 
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                                            Applikationshinweise
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                        Optimal working dilution should be determined by the investigator.
                                            
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                                            Plattentyp
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                        Pre-coated
                                            
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                                            Protokoll
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                        - Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
 
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                                            Beschränkungen
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                        Nur für Forschungszwecke einsetzbar
                                            
 
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                                            Haltbarkeit
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                        6 months
                                            
 
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                                                    - 
                        ATG4B
                                                    (Autophagy related 4B Cysteine Peptidase (ATG4B))
                                            
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                                            Andere Bezeichnung
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                        ATG4B
                                            
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                                            Gen-ID
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                        23192
                                            
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                                            UniProt
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                        Q9Y4P1
                                            
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                                            Pathways
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                        Autophagie
                                            
 
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