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ATG4B ELISA Kit
Human ATG4B ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human ATG4B in Cell Culture Supernatant, Cell Samples, Plasma, Serum und Tissue Lysate
Produktnummer ABIN6951529
Kurzübersicht für ATG4B ELISA Kit (ABIN6951529)
Target
Alle ATG4B Produkte
ATG4B
(Autophagy related 4B Cysteine Peptidase (ATG4B))
Reaktivität
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Proben
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktdetails ATG4B ELISA Kit
(ausblenden)
Verwendungszweck
Human ATG4B ELISA Kit.
Analytische Methode
Quantitative
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Anwendungsinformationen
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Applikationshinweise
Optimal working dilution should be determined by the investigator.
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Haltbarkeit
6 months
Details zu ATG4B
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ATG4B
(Autophagy related 4B Cysteine Peptidase (ATG4B))
Andere Bezeichnung
ATG4B
Gen-ID
23192
UniProt
Q9Y4P1
Pathways
Autophagie
Zuletzt angesehen
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