PFDN4 ELISA Kit

Produktnummer ABIN6951348

Produktdetails PFDN4 ELISA Kit

Target: PFDN4 (Prefoldin Subunit 4 (PFDN4))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human PFDN4 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu PFDN4

Target: PFDN4 (Prefoldin Subunit 4 (PFDN4))

Andere Bezeichnung: PFDN4 (PFDN4 Produkte)

Synonyme: DDBDRAFT_0204261 ELISA Kit, DDBDRAFT_0237717 ELISA Kit, DDB_0204261 ELISA Kit, DDB_0237717 ELISA Kit, pfd4 ELISA Kit, C1 ELISA Kit, PFD4 ELISA Kit, 0610025M10Rik ELISA Kit, 1810063B06Rik ELISA Kit, C-1 ELISA Kit, Pfd4 ELISA Kit, si:dkey-222e15.2 ELISA Kit, prefoldin subunit 4 ELISA Kit, prefoldin beta-like domain containing protein ELISA Kit, Prefoldin subunit 4 ELISA Kit, prefoldin 4 ELISA Kit, PFDN4 ELISA Kit, AFUA_1G15240 ELISA Kit, AOR_1_2080174 ELISA Kit, CMU_006340 ELISA Kit, pfdn4 ELISA Kit, pfd4 ELISA Kit, Pfdn4 ELISA Kit

Gen-ID: 5203

UniProt: Q9NQP4

Pathways: Unfolded Protein Response