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IFN alpha A ELISA Kit

Cow IFN alpha A ELISA-Kit Colorimetric-Assay zur Quantifizierung von Cow IFN alpha A in Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951055

Kurzübersicht für IFN alpha A ELISA Kit (ABIN6951055)

Target

Alle IFN alpha A (IFNAA) Produkte
IFN alpha A (IFNAA) (Interferon alpha-A (IFNAA))

Reaktivität

Rind (Kuh)

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Applikation

ELISA

Proben

Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
  • Verwendungszweck

    Bovine IFN-alpha A ELISA Kit.

    Analytische Methode

    Quantitative

    Produktmerkmale

    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    Bestandteile

    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate

    Benötigtes Material

    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Applikationshinweise

    Optimal working dilution should be determined by the investigator.

    Plattentyp

    Pre-coated

    Protokoll

    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Haltbarkeit

    6 months
  • Target Alle IFN alpha A (IFNAA) Produkte

    IFN alpha A (IFNAA) (Interferon alpha-A (IFNAA))

    Andere Bezeichnung

    IFN-alpha A

    Gen-ID

    515951

    UniProt

    P05007
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