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IL-5 IQ-ELISA Kit

IL5 Reaktivität: Human qPCR DNA-Binding ELISA 0.128 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN6730850
  • Target Alle IL-5 (IL5) IQ-ELISA Kits anzeigen
    IL-5 (IL5) (Interleukin 5 (IL5))
    Reaktivität
    • 11
    • 8
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Human
    Nachweismethode
    qPCR
    Methodentyp
    DNA-Binding ELISA
    Detektionsbereich
    0.128 pg/mL - 2000 pg/mL
    Untere Nachweisgrenze
    0.128 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Human Immunoquantitative (PCR-Based) IL-5 ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Proben
    Cell Culture Supernatant, Plasma, Serum
    Analytische Methode
    Quantitative
    Sensitivität
    0.128 pg/mL
    Produktmerkmale
    The Immuno Quantitative Enzyme Linked ImumunoSorbent Assay (IQELISA™) is an innovative new assay that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of lower sample volumes while also providing more sensitivity. The Human IL-5 IQELISA™ Kit is a modified ELISA assay with high sensitivity qPCR readout for the quantitative measurement of Human IL-5 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for Human IL-5 coated on a 96-well PCR plate. Standards and samples are pipetted into the wells and IL-5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and a detection affinity molecule is added to the plates. After washing away unbound detection affinity molecule, primers and a PCR master mix are added to the wells and data is collected using qPCR. Ct values obtained from the qPCR are then used to calculate the amount of antigen contained in each sample, where lower Ct values indicate a higher concentration of antigen.
    Bestandteile
    • IL-5 Microplate (Item A): 96 well PCR plate coated with anti-Human IL-5
    • Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
    • Standards (Item C): 2 vials of recombinant Human IL-5
    • Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
    • Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
    • Detection Affinity Reagent for IL-5 (Item F): 2 vials of a 4x concentrated solution of anti-Human IL-5 affinity reagent
    • IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
    • Primer Solution (Item I): 1.7 ml vial
    • PCR Master Mix (Item J): 1.2 ml vial
    • PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
    • Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer
    Benötigtes Material
    • Real-time PCR instrument, Bio-Rad recommended
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • 100 mL and 1 liter graduated cylinders
    • Absorbent paper
    • Distilled or deionized water
    • Log-log graph paper or computer and software for data analysis
    • Tubes to prepare standard or sample dilutions
    • Heating block or water bath capable of 80°C
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  • Applikationshinweise
    The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.
    Kommentare

    Features:
    - Excellent for follow up/confirmation of antibody array data
    - Pre-coated 96 well plate
    - Strip plate format minimizes waste
    - Complete kit includes all necessary reagents
    - 10x more sensitive than ELISA
    - Reproducible and reliable
    - Optimized for serum, plasma, urine and cell culture medium
    - 1/10th the sample volume of an ELISA

    Research Applications:
    - Detection of quantitative protein levels in biological fluids
    - Validation of antibody array results
    - Validation of biomarker discovery studies

    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed
    2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C
    3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
    4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
    5. Add 15μL Primer solution 10μL of PCR master mix to each well
    6. Run real-time PCR
    Aufbereitung der Reagenzien

    Recommended Dilution for serum and plasma samples: 2 - 10 fold

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Informationen zur Lagerung
    May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2°C to 8°C.
    Haltbarkeit
    6 months
  • Target Alle IL-5 (IL5) IQ-ELISA Kits anzeigen
    IL-5 (IL5) (Interleukin 5 (IL5))
    Andere Bezeichnung
    IL-5 (IL5 Produkte)
    Synonyme
    EDF IQ-ELISA Kit, IL-5 IQ-ELISA Kit, TRF IQ-ELISA Kit, Il-5 IQ-ELISA Kit, IL5 IQ-ELISA Kit, interleukin 5 IQ-ELISA Kit, IL5 IQ-ELISA Kit, Il5 IQ-ELISA Kit
    Gen-ID
    3567
    UniProt
    P05113
    Pathways
    JAK-STAT Signalweg, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
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