ACTH ELISA Kit

Produktnummer ABIN6730843

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Maus, Human und Ratte ACTH.

Kurzübersicht für ACTH ELISA Kit (ABIN6730843)

Target: ACTH (Adrenocorticotropic hormone (ACTH))

Reaktivität: Maus, Human, Ratte

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 0.1 ng/mL - 1000 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails ACTH ELISA Kit

Untere Nachweisgrenze: 0.1 ng/mL

Verwendungszweck: Human/Mouse/Rat Adrenocorticotropic Hormone EIA Kit optimized for serum and cell culture medium. Competition-based ELISA

Analytische Methode: Quantitative

Spezifität: This EIA kit is designed to detect human, mouse, and rat ACTH

Sensitivität: 0.5 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Anwendungsinformationen

Applikationshinweise: Suggested Plasma Dilution: Human: 2X / Mouse: 2X / Rat: 2X

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

• Prepare all reagents, samples and standards as instructed.
• Add 100 μL detection antibody to each well.
• Incubate 1.5 h at RT or O/N at 4 °C.
• Add 100 μL standard or sample to each well.
• Incubate 2.5 h at RT.
• Add 100 μL prepared streptavidin solution.
• Incubate 45 min at RT.
• Add 100 μL TMB One-Step Substrate Reagent to each well.
• Incubate 30 min at RT.
• Add 50 μL Stop Solution to each well.
• Read plate at 450 nm immediately.

Aufbereitung der Reagenzien:

Recommended Dilution for serum and plasma samplesHuman: 2x / Mouse: 2x / Rat: 2x

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Standard, Biotinylated ACTH peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Haltbarkeit: 6 months

Details zu ACTH

Target: ACTH (Adrenocorticotropic hormone (ACTH))

Andere Bezeichnung: Adrenocorticotropic hormone

Substanzklasse: Hormone

Hintergrund:

Gene Symbols: POMC

Background: Adrenocorticotropic hormone (ACTH, also adrenocorticotropin, corticotropin) is a 39 amino acids polypeptide tropic hormone. ACTH is synthesized from pre-pro-opiomelanocortin (pre-POMC), which is proteolytically cleaved by endopeptidases to yield various polypeptide fragments with varying physiological activity. ACTH is secreted by the anterior pituitary gland. It stimulates secretion of glucocorticoid steroid hormones from adrenal cortex cells, especially in the zona fasciculata of the adrenal glands. It influences steroid hormone secretion by both rapid short-term mechanisms and long-term actions. ACTH is also used as a medication and diagnostic agent.

Protein Name (Synonyms): Pro-opiomelanocortin (POMC) (Corticotropin-lipotropin) [Cleaved into: NPP, Melanotropin gamma (Gamma-MSH), Potential peptide, Corticotropin (Adrenocorticotropic hormone) (ACTH), Melanotropin alpha (Alpha-MSH), Corticotropin-like intermediary peptide (CLIP), Lipotropin beta (Beta-LPH), Lipotropin gamma (Gamma-LPH), Melanotropin beta (Beta-MSH), Beta-endorphin, Met-enkephalin]

Gen-ID: 5443

UniProt: P01189

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity