KIT ELISA Kit

Produktnummer ABIN6730842

Produktdetails KIT ELISA Kit

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Mouse CD117/c-kit ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Mouse CD117.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu KIT

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Andere Bezeichnung: CD117 (c-kit (KIT Produkte)

Synonyme: C-Kit ELISA Kit, CD117 ELISA Kit, PBT ELISA Kit, SCFR ELISA Kit, Bs ELISA Kit, Fdc ELISA Kit, Gsfsco1 ELISA Kit, Gsfsco5 ELISA Kit, Gsfsow3 ELISA Kit, SCO1 ELISA Kit, SCO5 ELISA Kit, SOW3 ELISA Kit, Ssm ELISA Kit, Tr-kit ELISA Kit, W ELISA Kit, c-KIT ELISA Kit, c-kit ELISA Kit, MGF ELISA Kit, KIT ELISA Kit, C-KIT ELISA Kit, kit ELISA Kit, receptor ELISA Kit, sparse ELISA Kit, Xkl-1 ELISA Kit, kit-a ELISA Kit, kit-b ELISA Kit, kl1-A ELISA Kit, krk1 ELISA Kit, pbt ELISA Kit, scfr ELISA Kit, KIT proto-oncogene receptor tyrosine kinase ELISA Kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog a ELISA Kit, KIT proto-oncogene receptor tyrosine kinase S homeolog ELISA Kit, KIT ELISA Kit, Kit ELISA Kit, kita ELISA Kit, kit ELISA Kit, kit.S ELISA Kit

UniProt: P05532

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity