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FZD4 ELISA Kit

FZD4 Reaktivität: Maus Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6730650
  • Target Alle FZD4 ELISA Kits anzeigen
    FZD4 (Frizzled Family Receptor 4 (FZD4))
    Reaktivität
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Mouse Frizzled-4 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes Mouse Frizzled-4.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle FZD4 ELISA Kits anzeigen
    FZD4 (Frizzled Family Receptor 4 (FZD4))
    Andere Bezeichnung
    Frizzled-4 (FZD4 Produkte)
    Synonyme
    CG4626 ELISA Kit, DFz4 ELISA Kit, Dfz4 ELISA Kit, Dm Fz4 ELISA Kit, Dmel\\CG4626 ELISA Kit, Fz4 ELISA Kit, anon-WO0170980.10 ELISA Kit, anon-WO0170980.11 ELISA Kit, fz1 ELISA Kit, zg01 ELISA Kit, CD344 ELISA Kit, EVR1 ELISA Kit, FEVR ELISA Kit, FZD4S ELISA Kit, Fz-4 ELISA Kit, FzE4 ELISA Kit, GPCR ELISA Kit, hFz4 ELISA Kit, frizzled4 ELISA Kit, fz4 ELISA Kit, FZ-4 ELISA Kit, frizzled 4 ELISA Kit, frizzled class receptor 4 ELISA Kit, frizzled-4 ELISA Kit, frizzled class receptor 4 S homeolog ELISA Kit, fz4 ELISA Kit, fzd4 ELISA Kit, Tsp_10376 ELISA Kit, FZD4 ELISA Kit, Fzd4 ELISA Kit, fzd4.S ELISA Kit
    UniProt
    Q61088
    Pathways
    WNT Signalweg, Hormone Transport, Sensory Perception of Sound
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