SFRP2 ELISA Kit

Produktnummer ABIN6730613

Das Maus SFRP2 ELISA Kit (ABIN6730613) ist ein Colorimetric ELISA Kit zur Detektion von Maus SFRP2.

Kurzübersicht für SFRP2 ELISA Kit (ABIN6730613)

Target: SFRP2 (Secreted Frizzled-Related Protein 2 (SFRP2))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.3 ng/mL - 300 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum

Produktdetails SFRP2 ELISA Kit

Untere Nachweisgrenze: 1.3 ng/mL

Verwendungszweck: Mouse sFRP-2 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytische Methode: Quantitative

Spezifität: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse A33, Angiogenin, ART4, ASGR1, BAMBI, Bcl-xL, BID, BMP7, CD160, CD34, CD5, CD74, CD99, CES1, CL-P1, CMG-2, DSPG3, EphA1, EphA7, EphB3, Erythropoietin, FAM3C, FDPS, FGF R4, Frizzled-9, GDF-8 Propeptide, IGSF4A, IL1F6, IL-5 R alpha, IP-10, JNK1, LTA4H, Mcpt1, MGL1, MMP7, Nectin-4, R-Spondin 1, Serpin A6, TNFb.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Suggested Plasma Dilution: 2 fold

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu SFRP2

Target: SFRP2 (Secreted Frizzled-Related Protein 2 (SFRP2))

Andere Bezeichnung: sFRP-2

Hintergrund:

Gene Symbols: Sarp1, Sdf5, Sfrp2

Protein Name (Synonyms): Secreted frizzled-related protein 2 (sFRP-2) (Protein SDF5) (Secreted apoptosis-related protein 1) (SARP-1)

Gen-ID: 20319

UniProt: P97299

Pathways: WNT Signalweg, Tube Formation, Positive Regulation of Endopeptidase Activity, Negative Regulation of intrinsic apoptotic Signaling, Positive Regulation of fat Cell Differentiation