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IGFBPI ELISA Kit
IGFBPI
Reaktivität: Maus
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6730494
Produktdetails IGFBPI ELISA Kit
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Target
Alle IGFBPI ELISA Kits anzeigen
IGFBPI
(Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))
Reaktivität
Alle Reaktivitäten für IGFBPI ELISA Kits
Maus
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Mouse IGFBP-1 ELISA Kit.
Proben
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes Mouse IGFBP-1.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Discover our top product IGFBPI ELISA Kit
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Anwendungsinformationen
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Applikationshinweise
Optimal working dilution should be determined by the investigator.
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu IGFBPI
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Target
Alle IGFBPI ELISA Kits anzeigen
IGFBPI
(Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))
Andere Bezeichnung
IGFBP-1 (IGFBPI Produkte )
Synonyme
cb656 ELISA Kit, igfbp1 ELISA Kit, IGFBP-1 ELISA Kit, MGC68538 ELISA Kit, afbp ELISA Kit, ibp1 ELISA Kit, pp12 ELISA Kit, igf-bp25 ELISA Kit, higfbp-1 ELISA Kit, IGFBP1 ELISA Kit, LOC100223208 ELISA Kit, LOC100305121 ELISA Kit, AFBP ELISA Kit, IBP1 ELISA Kit, IGF-BP25 ELISA Kit, PP12 ELISA Kit, hIGFBP-1 ELISA Kit, IGFBA ELISA Kit, insulin like growth factor binding protein 1 ELISA Kit, insulin-like growth factor binding protein 1a ELISA Kit, insulin like growth factor binding protein 1 S homeolog ELISA Kit, insulin like growth factor binding protein 1 L homeolog ELISA Kit, insulin-like growth factor binding protein 1 ELISA Kit, IGFBP1 ELISA Kit, igfbp1a ELISA Kit, igfbp1.S ELISA Kit, igfbp1.L ELISA Kit, igfbp1 ELISA Kit, LOC100305121 ELISA Kit, Igfbp1 ELISA Kit
UniProt
P47876
Pathways
Myometrial Relaxation and Contraction , ER-Nucleus Signaling , Growth Factor Binding
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