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IGFBPI ELISA Kit (Insulin-Like Growth Factor Binding Protein 1) ELISA Kit

IGFBPI Reaktivität: Maus AA 26-272 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Pubmed (3 Referenzen)
Produktnummer ABIN411289
$380.00
Zzgl. Versandkosten $45.00
96 tests
Lieferung in 4 bis 6 Werktagen
  • Target Alle IGFBPI ELISA Kits anzeigen
    IGFBPI
    Bindungsspezifität
    AA 26-272
    Reaktivität
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    31.2-2000 pg/mL
    Untere Nachweisgrenze
    31.2 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IGFBP-1
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: NSO
    Immunogen sequence: A26-N272
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <3pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: A26-N272
    Featured
    Zu unserem meistverkauften IGFBPI ELISA Kit
  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Kommentare

    Sequence similarities: Contains 1 IGFBP N-terminal domain.

    Plattentyp
    Pre-coated
    Protokoll
    mouse IGFBP-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IGFBP-1 has been precoated onto 96-well plates. Standards(NSO, A26-N272) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IGFBP-1 amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse IGFBP-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IGFBP-1 standard solution and each sample be measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(pg/ml): 108, Standard deviation: 5.72, CV(%): 5.3
    • Sample 2: n=16, Mean(pg/ml): 657, Standard deviation: 28.91, CV(%): 4.4
    • Sample 3: n=16, Mean(pg/ml): 1239, Standard deviation: 59.47, CV(%): 4.8,
    • Sample 1: n=24, Mean(pg/ml): 115, Standard deviation: 9.09, CV(%): 7.9
    • Sample 2: n=24, Mean(pg/ml): 638, Standard deviation: 41.47, CV(%): 6.5
    • Sample 3: n=24, Mean(pg/ml): 1341, Standard deviation: 97.89, CV(%): 7.3
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Matz-Soja, Aleithe, Marbach, Böttger, Arnold, Schmidt-Heck, Kratzsch, Gebhardt: "Hepatic Hedgehog signaling contributes to the regulation of IGF1 and IGFBP1 serum levels." in: Cell communication and signaling : CCS, Vol. 12, pp. 11, (2014) (PubMed).

    Zhang, Zhao, Hu, Liu, Chen, Chen, Du: "Distinct post-transcriptional regulation of Igfbp1 gene by hypoxia in lowland mouse and Qinghai-Tibet plateau root vole Microtus oeconomus." in: Molecular and cellular endocrinology, Vol. 376, Issue 1-2, pp. 33-42, (2013) (PubMed).

    Wang, Wang, Liang, Liu, Shi, Bai, Lin, Magaye, Zhao: "Expression and clinical significance of IGF-1, IGFBP-3, and IGFBP-7 in serum and lung cancer tissues from patients with non-small cell lung cancer." in: OncoTargets and therapy, Vol. 6, pp. 1437-44, (2013) (PubMed).

  • Target Alle IGFBPI ELISA Kits anzeigen
    IGFBPI
    Andere Bezeichnung
    IGFBP1 (IGFBPI Produkte)
    Synonyme
    cb656, igfbp1, IGFBP-1, MGC68538, afbp, ibp1, pp12, igf-bp25, higfbp-1, IGFBP1, LOC100223208, LOC100305121, AFBP, IBP1, IGF-BP25, PP12, hIGFBP-1, IGFBA, insulin like growth factor binding protein 1, insulin-like growth factor binding protein 1a, insulin like growth factor binding protein 1 S homeolog, insulin like growth factor binding protein 1 L homeolog, insulin-like growth factor binding protein 1, IGFBP1, igfbp1a, igfbp1.S, igfbp1.L, igfbp1, LOC100305121, Igfbp1
    Hintergrund

    Protein Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration (By similarity). .

    Background: IGFBP1 is also known as amniotic fluid binding protein(AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25. The low-molecular weight insulin-like growth-factor binding protein(IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth. These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II. The gene is organized in four exons and spans 5.9 kb. The IBP-1 gene is a single copy gene, located on chromosome 7. The standard product used in this kit is recombinant mouse IGFBP-1, consisting of 247 amino acids with the molecular mass of 27KDa.

    Synonyms: Insulin-like growth factor-binding protein 1,IBP-1,IGF-binding protein 1,IGFBP-1,Igfbp1,Igfbp-1,

    Full Gene Name: Insulin-like growth factor-binding protein 1

    Cellular Localisation: Secreted.
    Gen-ID
    16006
    UniProt
    P47876
    Pathways
    Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Growth Factor Binding
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