LAMP2 ELISA Kit

Produktnummer ABIN6730414

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Maus LAMP2.

Kurzübersicht für LAMP2 ELISA Kit (ABIN6730414)

Target: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 20.5 pg/mL - 6000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum

Produktdetails LAMP2 ELISA Kit

Untere Nachweisgrenze: 20.5 pg/mL

Verwendungszweck: Mouse LAMP2 (CD107b) ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytische Methode: Quantitative

Spezifität: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse ASAM, Cystatin B, DLL1, Kallikrein 7, Kremen-2, LIGHT, LIMP1, LRPAP, LRRC32, Matrilin-2, Mcpt6, MEP1A, MEPE, MESDC2, METRNL, Mimecan, Nectin-2, Neurturin, NGF R, NgR, Olfactomedin-1, Oncostatin M, OSM R beta, Osteoadherin, OX40, PD-1, PDGF R beta, PD-L2, PILRbeta, PLA2G2A, Plexin A1, Plexin C1, Podocalyxin, Podoplanin, Protocadherin-12, Prss34, RANK, Reg2, Relaxin1.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Suggested Plasma Dilution: 200 fold

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu LAMP2

Target: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Andere Bezeichnung: LAMP2 (CD107b

Hintergrund:

Gene Symbols: LAMP-2, LAMP2

Protein Name (Synonyms): Lysosome-associated membrane glycoprotein 2 (LAMP-2) (Lysosome-associated membrane protein 2) (CD107 antigen-like family member B) (Lysosomal membrane glycoprotein type B) (LGP-B) (CD antigen CD107b)

Gen-ID: 16784

UniProt: P17047

Pathways: Autophagie