Ephrin A5 ELISA Kit

Produktnummer ABIN6730156

Produktdetails Ephrin A5 ELISA Kit

Target: Ephrin A5 (EFNA5)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human Ephrin-A5 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human Ephrin-A5.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu Ephrin A5

Target: Ephrin A5 (EFNA5)

Andere Bezeichnung: Ephrin-A5 (EFNA5 Produkte)

Synonyme: EFNA5 ELISA Kit, af1 ELISA Kit, efl5 ELISA Kit, rags ELISA Kit, eplg7 ELISA Kit, lerk7 ELISA Kit, AL-1 ELISA Kit, AV158822 ELISA Kit, EFL-5 ELISA Kit, Ephrin-A5 ELISA Kit, Epl7 ELISA Kit, LERK-7 ELISA Kit, RAGS ELISA Kit, Lerk7 ELISA Kit, AF1 ELISA Kit, EFL5 ELISA Kit, EPLG7 ELISA Kit, GLC1M ELISA Kit, LERK7 ELISA Kit, ephrin A5 ELISA Kit, EFNA5 ELISA Kit, efna5 ELISA Kit, Efna5 ELISA Kit

UniProt: P52803

Pathways: RTK Signalweg