Angiostatin ELISA Kit

Produktnummer ABIN6720398

Hersteller Produkt- Nr.: koa0545

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human Angiostatin in Cell Culture Supernatant, Plasma und Serum.

Kurzübersicht für Angiostatin ELISA Kit (ABIN6720398)

Target: Angiostatin (ANG)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum

Produktdetails Angiostatin ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Hersteller Produkt- Nr.: koa0545

Hersteller: Rockland

Verwendungszweck: Human Angiostatin K1-3 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human Angiostatin K1-3. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Angiostatin K1-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human serum, plasma or cell culture supernates to each empty well. It is recommended that each human Angiostatin K1-3 standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: NSO, Immunogen sequence: V98-S355(K1-3)

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Angiostatin

Target: Angiostatin (ANG)

Andere Bezeichnung: Angiostatin K1-3

Hintergrund:

Synonyms: Ang K1-3

Background: Ang K1-3 is a single, non-glycosylated polypeptide chain containing 259 amino acids. It represents a proteolytic fragment of plasminogen containing the first three kringle structures. Ang K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. It displays increased inhibitory activity(ED₅₀ =70 nM) relative to kringles 1-4(ED₅₀ = 135 nM).

Gen-ID: 5340

NCBI Accession: NP_000292

UniProt: P00747

Pathways: Lipid Metabolism