APOE ELISA Kit

Produktnummer ABIN6720363

Produktdetails APOE ELISA Kit

Target: APOE (Apolipoprotein E (APOE))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 3.12 ng/mL - 200 ng/mL

Untere Nachweisgrenze: 3.12 ng/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Milk, Plasma (EDTA - heparin), Saliva, Serum, Urine

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: AD2, Alzheimer disease 2, Apo E, Apo-E, ApoE, APOEA, Apolipoprotein E, Apolipoprotein-E, ApolipoproteinE, Apoprotein, LDLCQ5, LPG

Background: Apolipoprotein E (APOE) is a class of apolipoprotein found in the chylomicron and Intermediate-density lipoprotein (IDLs) that is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. In peripheral tissues, APOE is primarily produced by the liver and macrophages, and mediates cholesterol metabolism in an isoform- dependent manner. In the central nervous system, APOE is mainly produced by astrocytes, and transports cholesterol to neurons via APOE receptors, which are members of the low density lipoprotein receptor gene family. This protein is involved in Alzheimer's disease and cardiovascular disease.

Gene Name: APOE

Production: Natural human APOE. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: K19-H317

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 200 ng/mL, 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL human APOE standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva, milk or urine to each empty well. It is recommended that each human APOE standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 3.12 ng/mL-200 ng/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu APOE

Target: APOE (Apolipoprotein E (APOE))

Andere Bezeichnung: APOE (APOE Produkte)

Synonyme: ad2 ELISA Kit, apoprotein ELISA Kit, im:7036787 ELISA Kit, wu:fb69a05 ELISA Kit, zgc:110064 ELISA Kit, apoe ELISA Kit, AI255918 ELISA Kit, AD2 ELISA Kit, LDLCQ5 ELISA Kit, LPG ELISA Kit, APOEA ELISA Kit, Apo-E ELISA Kit, apolipoprotein E ELISA Kit, apolipoprotein Ea ELISA Kit, apoe ELISA Kit, apoea ELISA Kit, Apoe ELISA Kit, APOE ELISA Kit

Gen-ID: 348

NCBI Accession: NP_000032

UniProt: P02649

Pathways: Regulation of Cell Size, Lipid Metabolism