Clusterin ELISA Kit

Produktnummer ABIN6720361

Hersteller Produkt- Nr.: koa0551

Human Clusterin ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human Clusterin.

Kurzübersicht für Clusterin ELISA Kit (ABIN6720361)

Target: Clusterin (CLU)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78 ng/mL - 50 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum, Urine

Produktdetails Clusterin ELISA Kit

Untere Nachweisgrenze: 0.78 ng/mL

Hersteller Produkt- Nr.: koa0551

Hersteller: Rockland

Verwendungszweck: Human Clusterin Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human Clusterin. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 20 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Clusterin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva or urine to each empty well. We recommend that each human Clusterin standard solution and each sample is measured in duplicate.

Kommentare:

Standard: Expression system for standard: NSO, Immunogen sequence: D23-R227(beta)&S228-E449(alpha)

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Clusterin

Target: Clusterin (CLU)

Andere Bezeichnung: Clusterin

Hintergrund:

Synonyms: AAG4, Aging associated protein 4, APO J, APOJ, CLI, CLU, CLU1, CLU2, Clusterin, Complement associated protein SP 40,40, Complement cytolysis inhibitor, Complement lysis inhibitor, Ku70 binding protein 1, KUB1, NA1/NA2, SGP 2, SGP2, SP 40, SP40, Sulfated glycoprotein 2, Testosterone repressed prostate message 2, TRPM 2, TRPM2

Background: Clusterin (apolipoprotein J) is a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis.[1] In humans, clusterin is encoded by the CLU gene. This protein has several synonyms: dimeric acidic glycoprotein(DAG protein), testosterone repressed prostate message-2(TRPM-2), sulfated glycoprotein-2(SGP-2) and complement lysis inhibitor(CLI). Clusterin was mapped to mouse chromosome 14. Clusterin is a ubiquitously expressed molecule thought to influence a variety of processes including cell death. In the brain, it accumulates in dying neurons following seizures and hypoxic-ischemic(H-I) injury. clusterin may be a new therapeutic target to modulate non-caspase-dependent neuronal death following acute brain injury.

Gen-ID: 1191

NCBI Accession: NP_001822

UniProt: P10909

Pathways: Apoptose, Negative Regulation of intrinsic apoptotic Signaling