RBP4 ELISA Kit

Produktnummer ABIN6720318

Hersteller Produkt- Nr.: koa0498

Dieses Human RBP4 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human RBP4 zu quantifizieren.

Kurzübersicht für RBP4 ELISA Kit (ABIN6720318)

Target: RBP4 (Retinol Binding Protein 4, Plasma (RBP4))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 312 pg/mL - 20000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Urine

Produktdetails RBP4 ELISA Kit

Untere Nachweisgrenze: 312 pg/mL

Hersteller Produkt- Nr.: koa0498

Hersteller: Rockland

Verwendungszweck: Human RBP4 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human RBP4. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human RBP4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA) or urine to each empty well. We recommend that each human RBP4 standard solution and each sample is measured in duplicate.

Kommentare:

Standard: Expression system for standard: NSO, Immunogen sequence: E19-L201

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu RBP4

Target: RBP4 (Retinol Binding Protein 4, Plasma (RBP4))

Andere Bezeichnung: RBP4

Hintergrund:

Synonyms: Plasma retinol binding protein, Plasma retinol-binding protein, Plasma retinol-binding protein(1-176), PRBP, PRO2222, RBP, RBP4, RDCCAS, RET4_HUMAN, Retinol binding protein 4, Retinol binding protein 4 interstitial, Retinol binding protein 4 plasma

Background: Retinol binding protein 4, plasma, also known as RBP4, belongs to the lipocalin family and is the specific carrier for retinol(vitamin A alcohol) in the blood. It is protein that in humans is encoded by the RBP4 gene. RBP4 gene resides just centromeric of the cluster of CYP2C genes on 10q24. The mouse Rbp4 locus is closely linked and just proximal to the locus for phenobarbital-inducible cytochrome P450-2c(Cyp-2c) at the distal end of chromosome 19. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein post translationally and results in defective delivery and supply to the epidermal cells. The standard used in this kit is recombinant protein, with E19-L201 aa sequence, the molecular weight is 22kda.

Gen-ID: 5950

NCBI Accession: NP_006735

UniProt: P02753

Pathways: Regulatorische RNA Pathways, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response