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Angiotensin I Converting Enzyme 1 ELISA Kit

Maus Angiotensin I Converting Enzyme 1 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus Angiotensin I Converting Enzyme 1 in Cell Culture Supernatant, Plasma (heparin) und Serum
Rockland
Produktnummer ABIN6720307
Hersteller Produkt- Nr.: koa0338

Kurzübersicht für Angiotensin I Converting Enzyme 1 ELISA Kit (ABIN6720307)

Target

Alle Angiotensin I Converting Enzyme 1 (ACE) ELISA Kits anzeigen
Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

Reaktivität

  • 7
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Maus

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

93.8 pg/mL - 6000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Plasma (heparin), Serum
  • Untere Nachweisgrenze

    93.8 pg/mL

    Hersteller Produkt- Nr.

    koa0338

    Hersteller

    Rockland

    Verwendungszweck

    Mouse ACE Sandwich ELISA Kit for Quantitative Detection

    Marke

    AccuSignal™

    Analytische Methode

    Quantitative

    Spezifität

    Production: Natural and recombinant mouse ACE. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    < 10 pg/mL

    Bestandteile

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • Applikationshinweise

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 419pg/mL, 187.5pg/mL, 93.8pg/mL mouse ACE standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin) to each empty well. We recommend that each mouse ACE standard solution and each sample is measured in duplicate.

    Kommentare

    Standard: Expression system for standard: NSO, Immunogen sequence: L35-Q1264

    Probenmenge

    100 μL

    Plattentyp

    Pre-coated

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    Haltbarkeit

    12 months
  • Target Alle Angiotensin I Converting Enzyme 1 (ACE) ELISA Kits anzeigen

    Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

    Andere Bezeichnung

    ACE

    Hintergrund

    Synonyms: Angiotensin converting enzyme, CD143, DCP, Dcp1, Dipeptidyl carboxypeptidase I, Kininase II

    Background: Angiotensin-converting enzyme(ACE), an exopeptidase, is a circulating enzyme that participates in the body's renin-angiotensin system(RAS), which mediates extracellular volume(i.e. that of the blood plasma, lymph and interstitial fluid), and arterial vasoconstriction. It is secreted by pulmonary and renal endothelial cells and catalyzes the conversion of decapeptide angiotensin I to octapeptide angiotensin II. Using a DNA marker at the growth hormone gene locus, which they characterized as 'extremely polymorphic' and which showed no recombination with ACE, ACE was mapped to 17q22-q24, consistent with the in situ hybridization mapping to 17q23. ACE, or kininase II, is a dipeptidyl carboxypeptidase that plays an important role in blood pressure regulation and electrolyte balance by hydrolyzing angiotensin I into angiotensin II, a potent vasopressor, and aldosterone-stimulating peptide. The enzyme is also able to inactivate bradykinin, a potent vasodilator.

    Gen-ID

    11421

    NCBI Accession

    NP_033728

    UniProt

    P09470

    Pathways

    ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration
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