Ficolin 2 ELISA Kit

Produktnummer ABIN6720298

Hersteller Produkt- Nr.: koa0742

Human Ficolin 2 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human Ficolin 2.

Kurzübersicht für Ficolin 2 ELISA Kit (ABIN6720298)

Target: Ficolin 2 (FCN2) (Ficolin (Collagen/fibrinogen Domain Containing Lectin) 2 (Hucolin) (FCN2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78 ng/mL - 50 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (heparin), Serum

Produktdetails Ficolin 2 ELISA Kit

Untere Nachweisgrenze: 0.78 ng/mL

Hersteller Produkt- Nr.: koa0742

Hersteller: Rockland

Verwendungszweck: Human Ficolin-2 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NS0
Immunogen sequence: L26-A313

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 20 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Kommentare:

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Ficolin-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each human Ficolin-2 standard solution and each sample be measured in duplicate.

Testpräzision: Intra-Assay Precision (CV%): 4.6%, 7.3%, 6.1%
Inter-Assay Precision (CV%): 5.4%, 7.8%, 7.7%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Ficolin 2

Target: Ficolin 2 (FCN2) (Ficolin (Collagen/fibrinogen Domain Containing Lectin) 2 (Hucolin) (FCN2))

Andere Bezeichnung: Ficolin-2

Hintergrund:

Background: Ficolin-2, which was initially identified as L-ficolin, is a protein that in humans is encoded by the FCN2 gene. The product of this gene belongs to the ficolin family of proteins. This family is characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. It is mapped to 9q34.3. This gene is predominantly expressed in the liver, and has been shown to have carbohydrate binding and opsonic activities. Serum concentration of ficolin-2 varies considerably in healthy individuals. FCN2 promoter polymorphisms were associated with marked changes in theficolin-2 serum concentration.

Gene Full Name: ficolin 2

Gen-ID: 2220

NCBI Accession: NP_004099

UniProt: Q15485

Pathways: Komplementsystem