CCL21 ELISA Kit

Produktnummer ABIN6720217

Produktdetails CCL21 ELISA Kit

Target: CCL21 (Chemokine (C-C Motif) Ligand 21 (CCL21))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: 6Ckine, Beta chemokine exodus 2, C-C motif chemokine 21, Chemokine (C-C motif) ligand 21, CKb9, ECL, Efficient Chemoattractant for Lymphocytes, Exodus2, MGC34555, OTTHUMP00000000526, OTTHUMP00000000527, OTTHUMP00000021303, SCYA 21, SCYA21, Secondary lymphoid tissue chemokine, SLC, Small inducible cytokine A21, Small inducible cytokine subfamily A (Cys-Cys), member 21, TCA 4, TCA4

Background: Chemokine(C-C motif) ligand 21(CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine(because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine(SLC). By somatic cell hybrid and radiation hybrid analyses, mapped the SCYA21 gene to 9p13. Chemokines are a family of proteins that direct leukocyte migration and activation to inflammatory stimuli. CXC chemokine ligand 13(CXCL13), CC chemokine ligand 21(CCL21), and CCL19 are constitutively expressed in secondary lymphoid organs, where they control the placement of lymphocytes and dendritic cells. It was demonstrate that the local expression of homeostatic chemokines in nonlymphoid organs, such as the lung, plays an important role in protective immune responses.

Gene Name: CCL21

Production: Natural and recombinant human CCL21. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: S24-P134

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human CCL21 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CCL21 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu CCL21

Target: CCL21 (Chemokine (C-C Motif) Ligand 21 (CCL21))

Andere Bezeichnung: CCL21 - 6Ckine (CCL21 Produkte)

Synonyme: 6Ckine ELISA Kit, CKb9 ELISA Kit, ECL ELISA Kit, SCYA21 ELISA Kit, SLC ELISA Kit, TCA4 ELISA Kit, ccl21 ELISA Kit, Ccl21b ELISA Kit, C-C motif chemokine ligand 21 ELISA Kit, C-C motif chemokine 21 ELISA Kit, CCL21 ELISA Kit, ccl21 ELISA Kit, Ccl21 ELISA Kit

Gen-ID: 6366

NCBI Accession: NP_002980

UniProt: O00585

Pathways: Regulation of Actin Filament Polymerization