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CCL21 ELISA Kit

CCL21 Reaktivität: Human AA 24-134 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Produktnummer ABIN1672754
  • Target Alle CCL21 ELISA Kits anzeigen
    CCL21 (Chemokine (C-C Motif) Ligand 21 (CCL21))
    Bindungsspezifität
    AA 24-134
    Reaktivität
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    62.5-4000 pg/mL
    Untere Nachweisgrenze
    62.5 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CCL21/6Ckine
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: E.coli
    Immunogen sequence: S24-P134
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <12pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: E.coli
    Immunogen sequence: S24-P134
    Featured
    Zu unserem meistverkauften CCL21 ELISA Kit
  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Kommentare

    Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.

    Tissue Specificity: Highly expressed in high endothelial venules of lymph nodes, spleen and appendix. Intermediate levels found in small intestine, thyroid gland and trachea. Low level expression in thymus, bone marrow, liver, and pancreas. Also found in tonsil, fetal heart and fetal spleen.

    Plattentyp
    Pre-coated
    Protokoll
    human CCL21 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL21 has been precoated onto 96-well plates. Standards(E.coli, S24-P134) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL21 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL21 amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human CCL21 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CCL21 standard solution and each sample be measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(pg/ml): 425, Standard deviation: 20.83, CV(%): 4.9
    • Sample 2: n=16, Mean(pg/ml): 1121, Standard deviation: 43.72, CV(%): 3.9
    • Sample 3: n=16, Mean(pg/ml): 2397, Standard deviation: 110.3, CV(%): 4.6,
    • Sample 1: n=24, Mean(pg/ml): 436, Standard deviation: 32.26, CV(%): 7.4
    • Sample 2: n=24, Mean(pg/ml): 1247, Standard deviation: 77.31, CV(%): 6.2
    • Sample 3: n=24, Mean(pg/ml): 2438, Standard deviation: 163.4, CV(%): 6.7
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Chen, Luo, Wen, Li, Li, Wang, Du, Zhang, Tang, Yang, Hu, Zhao, Wei: "Low-dose paclitaxel improves the therapeutic efficacy of recombinant adenovirus encoding CCL21 chemokine against murine cancer." in: Cancer science, Vol. 105, Issue 11, pp. 1393-401, (2015) (PubMed).

  • Target Alle CCL21 ELISA Kits anzeigen
    CCL21 (Chemokine (C-C Motif) Ligand 21 (CCL21))
    Andere Bezeichnung
    CCL21 (CCL21 Produkte)
    Synonyme
    6Ckine ELISA Kit, CKb9 ELISA Kit, ECL ELISA Kit, SCYA21 ELISA Kit, SLC ELISA Kit, TCA4 ELISA Kit, ccl21 ELISA Kit, Ccl21b ELISA Kit, C-C motif chemokine ligand 21 ELISA Kit, C-C motif chemokine 21 ELISA Kit, CCL21 ELISA Kit, ccl21 ELISA Kit, Ccl21 ELISA Kit
    Hintergrund

    Protein Function: Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.

    Background: Chemokine(C-C motif) ligand 21(CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine(because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine(SLC). By somatic cell hybrid and radiation hybrid analyses, mapped the SCYA21 gene to 9p13. Chemokines are a family of proteins that direct leukocyte migration and activation to inflammatory stimuli. CXC chemokine ligand 13(CXCL13), CC chemokine ligand 21(CCL21), and CCL19 are constitutively expressed in secondary lymphoid organs, where they control the placement of lymphocytes and dendritic cells. It was demonstrate that the local expression of homeostatic chemokines in nonlymphoid organs, such as the lung, plays an important role in protective immune responses.

    Synonyms: C-C motif chemokine 21,6Ckine,Beta-chemokine exodus-2,Secondary lymphoid-tissue chemokine,SLC,Small-inducible cytokine A21,CCL21,SCYA21,UNQ784/PRO1600,

    Full Gene Name: C-C motif chemokine 21

    Cellular Localisation: Secreted.
    Gen-ID
    6366
    UniProt
    O00585
    Pathways
    Regulation of Actin Filament Polymerization
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