CSF1R ELISA Kit

Produktnummer ABIN6720209

Hersteller Produkt- Nr.: koa0479

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus CSF1R in Cell Culture Supernatant, Plasma (EDTA - heparin) und Serum.

Kurzübersicht für CSF1R ELISA Kit (ABIN6720209)

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Produktdetails CSF1R ELISA Kit

Untere Nachweisgrenze: 62.5 pg/mL

Hersteller Produkt- Nr.: koa0479

Hersteller: Rockland

Verwendungszweck: Mouse CSF1R - M-CSFR Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant mouse CSF1R. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 62.5 pg/mL-4000 pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse CSF1R standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CSF1R standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: NSO, Immunogen sequence: A20-S511

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu CSF1R

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Andere Bezeichnung: CSF1R/M-CSFR

Hintergrund:

Synonyms: C FMS, CD 115, CD115, CD115 antigen, CFMS, Colony stimulating factor 1 receptor, CSF-1 receptor, CSF-1-R, CSF1R_HUMAN, CSFR, FIM2, FMS, FMS proto oncogene, FMS protooncogene, HDLS, M-CSF Receptor, M-CSF-R, Macrophage colony stimulating factor 1 receptor, Macrophage colony-stimulating factor 1 receptor, McDonough feline sarcoma viral (v fms) oncogene homolog, Oncogen FMS, Proto-oncogene c-Fms, V-FMS McDonough feline sarcoma viral oncogen homolog

Background: CSF1R(Colony stimulating factor 1 receptor),also known as M-CSFR and CD115, is a cell-surface protein encoded, in humans, by the CSF1R gene. The gene is located on long arm of chromosome 5(5q32) on the Crick(minus) strand. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. The encoded protein is a single pass type I membrane protein and acts as the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. Both CSF1R, and its ligand colony stimulating factor 1 play an important role in the development of the mammary gland and may be involved in the process of mammary gland carcinogenesis.

Gen-ID: 12978

NCBI Accession: NP_001032948

UniProt: P09581

Pathways: RTK Signalweg, Inositol Metabolic Process, Cell-Cell Junction Organization