Leptin Receptor ELISA Kit

Produktnummer ABIN6720133

Hersteller Produkt- Nr.: koa0249

Maus Leptin Receptor ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus Leptin Receptor.

Kurzübersicht für Leptin Receptor ELISA Kit (ABIN6720133)

Target: Leptin Receptor (LEPR)

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Produktdetails Leptin Receptor ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Hersteller Produkt- Nr.: koa0249

Hersteller: Rockland

Verwendungszweck: Mouse Leptin receptor Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant mouse Leptin receptor. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 5 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Leptin receptor standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse Leptin receptor standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: NSO, Immunogen sequence:  L22-G839

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Leptin Receptor

Target: Leptin Receptor (LEPR)

Andere Bezeichnung: Leptin receptor

Hintergrund:

Synonyms: CD295, HuB220, LEP-R, LEPR, LEPR_HUMAN, Leptin receptor, OB receptor, OB-R, OBR

Background: Leptin receptor(or Obese receptor, OBR) is a single membrane-spanning receptor most related to the gp130 signal-transducing component of the IL-6 receptor, the G-CSF receptor, and the LIF receptor. OB-R mRNA is expressed not only in choroid plexus, but also in several other tissues, including hypothalamus. Leptin acts through the leptin receptor, a single-transmembrane domain receptor of the cytokine-receptor family. Leptin controls energy balance and food intake through the leptin receptor in the hypothalamus of the brain, which suggests that some polymorphisms of the leptin receptor gene(LEPR) might contribute to obesity or obesity-related diseases. Leptin is also involved in the regulation of blood pressure through the leptin receptor.

Gen-ID: 16847

NCBI Accession: NP_001116371

UniProt: P48356

Pathways: JAK-STAT Signalweg, AMPK Signaling, Feeding Behaviour