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Cathepsin B ELISA Kit

Dieses Human Cathepsin B ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human Cathepsin B zu quantifizieren.
Rockland
Produktnummer ABIN6720096
Hersteller Produkt- Nr.: koa0385

Kurzübersicht für Cathepsin B ELISA Kit (ABIN6720096)

Target

Alle Cathepsin B (CTSB) ELISA Kits anzeigen
Cathepsin B (CTSB)

Reaktivität

  • 5
  • 4
  • 2
  • 1
  • 1
  • 1
Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

156 pg/mL - 10000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • Untere Nachweisgrenze

    156 pg/mL

    Hersteller Produkt- Nr.

    koa0385

    Hersteller

    Rockland

    Verwendungszweck

    Human Cathepsin B Sandwich ELISA Kit for Quantitative Detection

    Marke

    AccuSignal™

    Analytische Methode

    Quantitative

    Spezifität

    Production: Natural and recombinant human Cathepsin B. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    < 5 pg/mL

    Bestandteile

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • Applikationshinweise

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Cathepsin B standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Cathepsin B standard solution and each sample be measured in duplicate.

    Kommentare

    Standard: Expression system for standard: NSO, Immunogen sequence: R18-I339

    Probenmenge

    100 μL

    Plattentyp

    Pre-coated

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    Haltbarkeit

    12 months
  • Target Alle Cathepsin B (CTSB) ELISA Kits anzeigen

    Cathepsin B (CTSB)

    Andere Bezeichnung

    Cathepsin B

    Hintergrund

    Synonyms: aAmyloid precursor protein secretase, APP secretase, APPS, CATB_HUMAN, Cathepsin B heavy chain, Cathepsin B1, CPSB, CTSB, Cysteine protease

    Background: Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene.1, 2 And this gene is mapped to chromosome 8p22.3 The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease.4 Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant human Cathepsin B with the molecular mass of 37KDa.

    Gen-ID

    1508

    NCBI Accession

    NP_001899

    UniProt

    P07858

    Pathways

    Activation of Innate immune Response, Toll-Like Receptors Cascades
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