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HBEGF ELISA Kit

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human HBEGF.
Rockland
Produktnummer ABIN6720047
Hersteller Produkt- Nr.: koa0456

Kurzübersicht für HBEGF ELISA Kit (ABIN6720047)

Target

Alle HBEGF ELISA Kits anzeigen
HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))

Reaktivität

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Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

15.6 pg/mL - 1000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • Untere Nachweisgrenze

    15.6 pg/mL

    Hersteller Produkt- Nr.

    koa0456

    Hersteller

    Rockland

    Verwendungszweck

    Human HBEGF Sandwich ELISA Kit for Quantitative Detection

    Marke

    AccuSignal™

    Analytische Methode

    Quantitative

    Spezifität

    Production: Natural and recombinant human HBEGF. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    < 10 pg/mL

    Bestandteile

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • Applikationshinweise

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human HBEGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human HBEGF standard solution and each sample be measured in duplicate.

    Kommentare

    Standard: Expression system for standard: sf21, Immunogen sequence: D63-L148

    Probenmenge

    100 μL

    Plattentyp

    Pre-coated

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    Haltbarkeit

    12 months
  • Target Alle HBEGF ELISA Kits anzeigen

    HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))

    Andere Bezeichnung

    HBEGF

    Hintergrund

    Synonyms: Diphtheria toxin receptor, diphtheria toxin receptor (heparin-binding EGF-like growth factor), diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor), DT R, DT-R, DTR, DTS, DTSF, HB-EGF, HBEGF, HBEGF_HUMAN, HEGFL, Heparin binding EGF like growth factor, Heparin binding epidermal growth factor, Heparin binding epidermal growth factor like growth factor, Heparin-binding EGF-like growth factor, Proheparin binding EGF like growth factor

    Background: HBEGF(Heparin-binding EGF-like growth factor), also known as HEGFL or DTR, is a member of the EGF family of proteins that in humans is encoded by the HBEGF gene. The HBEGF gene is assigned to chromosome 5, thus confirming the assignment of the gene on the basis of its role in relation to diphtheria toxin susceptibility. HB-EGF is an 87 amino acid glycoprotein which displays highly regulated gene expression. It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function. HB-EGF binding and activation of EGF receptors plays a critical role during cardiac valve tissue development and the maintenance of normal heart function in adults.

    Gen-ID

    1839

    NCBI Accession

    NP_001936

    UniProt

    Q99075

    Pathways

    RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung
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