Thrombospondin 1 ELISA Kit

Produktnummer ABIN6720022

Produktdetails Thrombospondin 1 ELISA Kit

Target: Thrombospondin 1 (THBS1)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56 ng/mL - 100 ng/mL

Untere Nachweisgrenze: 1.56 ng/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: THBS 1, THBS, THBS1, Thrombospondin 1, Thrombospondin-1, Tsp 1, TSP, Tsp1, TSP1_HUMAN

Background: Thrombospondin 1, also known as THBS1, is a protein that in humans in encoded by the THBS1 gene. Thrombospondin 1 is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. By in situ hybridization, the THBS1 gene was mapped to human 15q15 and the cognate gene to mouse chromosome 2(region F) and was localized to 15q11-qter by Southern analysis of human-rodent somatic cell hybrids. Thrombospondin I is a multimodular secreted protein that associates with the extracellular matrix and possesses a variety of biologic functions, including a potent antiangiogenic activity. Other thrombospondin genes include thrombospondins II, III, and IV.

Gene Name: THBS1

Production: Natural and recombinant human THBS1. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: N19-P1170

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human THBS1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human THBS1 standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 1.56 ng/mL-100 ng/mL

Other Performance Data: DOE: 2-8° C - 03/18/19 -20° C - 09/06/19

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu Thrombospondin 1

Target: Thrombospondin 1 (THBS1)

Andere Bezeichnung: THBS1 - TSP1 (THBS1 Produkte)

Synonyme: THBS ELISA Kit, THBS-1 ELISA Kit, TSP ELISA Kit, TSP-1 ELISA Kit, TSP1 ELISA Kit, Thbs-1 ELISA Kit, tbsp1 ELISA Kit, thrombospondin-1 ELISA Kit, thbs ELISA Kit, thbs-1 ELISA Kit, tsp ELISA Kit, tsp-1 ELISA Kit, tsp1 ELISA Kit, Tsp1 ELISA Kit, THBS1 ELISA Kit, Tsp-1 ELISA Kit, wu:fc48c03 ELISA Kit, si:dkey-11e23.1 ELISA Kit, tsp2 ELISA Kit, thrombospondin 1 ELISA Kit, thrombospondin 1 S homeolog ELISA Kit, thrombospondin 1b ELISA Kit, thrombospondin 2 L homeolog ELISA Kit, THBS1 ELISA Kit, Thbs1 ELISA Kit, thbs1.S ELISA Kit, thbs1b ELISA Kit, thbs1 ELISA Kit, thbs2.L ELISA Kit

Gen-ID: 7057

NCBI Accession: NP_003237

UniProt: P07996

Pathways: Autophagie