Complement Factor H ELISA Kit

Produktnummer ABIN6720016

Hersteller Produkt- Nr.: koa0844

Maus Complement Factor H ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus Complement Factor H.

Kurzübersicht für Complement Factor H ELISA Kit (ABIN6720016)

Target: Complement Factor H (CFH)

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78 ng/mL - 50 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Produktdetails Complement Factor H ELISA Kit

Untere Nachweisgrenze: 0.78 ng/mL

Hersteller Produkt- Nr.: koa0844

Hersteller: Rockland

Verwendungszweck: Mouse Complement H - CFH Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant mouse CFH. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 20 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 0.78 ng/mL-50 ng/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL mouse CFH standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse CFH standard solution and each sample is measured in duplicate.

Kommentare:

Standard: Expression system for standard: NSO, Immunogen sequence: S875-V1252

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Complement Factor H

Target: Complement Factor H (CFH)

Andere Bezeichnung: Complement H/CFH

Hintergrund:

Synonyms: adrenomedullin binding protein, age related maculopathy susceptibility 1, AHUS 1, AHUS1, AMBP 1, AMBP1, ARMD 4, ARMD4, ARMS 1, ARMS1, beta 1 H globulin, beta 1H, beta1H, CFAH_HUMAN, CFH, CFHL 3, CFHL3, Complement factor H, complement factor H isoform b, Factor H, factor H like 1, FH, FHL 1, FHL1, H factor 1 (complement)|H factor 1, H factor 2 (complement), HF 1, HF 2, HF, HF1, HF2, HUS, MGC88246

Background: Complement factor H (CFH), originally known as beta-1H globulin, is a serum glycoprotein that regulates the function of the alternative complement pathway in fluid phase and on cellular surfaces. It binds to C3b, accelerates the decay of the alternative pathway convertase C3bBb, and also acts as a cofactor for complement factor I, another C3b inhibitor. The CFH gene is located on chromosome 1q32-q32.1 within a cluster of genes encoding the regulatory complement components of the activation of C3 (RCA for 'regulators of complement activation'). This gene cluster includes decay-accelerating factor (DAF), C4-binding protein (C4BPA and C4BPB), and the factor H-related genes CFHR1, CFHR2, CFHR3, CFHR4, and CFHR5, among others. The gene family has arisen by multiple duplication events.

Gen-ID: 12628

NCBI Accession: NP_034018

UniProt: P06909

Pathways: Komplementsystem, Cellular Response to Molecule of Bacterial Origin