CCL3 ELISA Kit

Produktnummer ABIN6720004

Hersteller Produkt- Nr.: koa0258

Maus CCL3 ELISA Kit, Colorimetric Assay für die Quantifizierung von Maus CCL3 und es wurde in 2+ Publikationen zitiert.

Kurzübersicht für CCL3 ELISA Kit (ABIN6720004)

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.8 pg/mL - 500 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum

Produktdetails CCL3 ELISA Kit

Untere Nachweisgrenze: 7.8 pg/mL

Hersteller Produkt- Nr.: koa0258

Hersteller: Rockland

Verwendungszweck: Mouse CCL3 - MIP1 alpha Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: A24-A92

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Kommentare:

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL mouse CCL3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. We recommend that each mouse CCL3 standard solution and each sample is measured in duplicate.

Testpräzision: Intra-Assay Precision (CV%): 4.1%, 5.4%, 4%
Inter-Assay Precision (CV%): 4.2%, 7.2%, 5.8%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Referenzen für CCL3 ELISA Kit (ABIN6720004)

Wang, Huang, Lu, Liu, Mo: "Immuno-protective vesicle-crosslinked hydrogel for allogenic transplantation." in: Nature communications, Vol. 15, Issue 1, pp. 5176, (2024) (PubMed).

Nian, Li, Ma, Li, Li, Zhang, Ohiolei, Li, Dai, Liu, Yan, Fu, Xiao, Jia: "Understanding pathogen-host interplay by expression profiles of lncRNA and mRNA in the liver of Echinococcus multilocularis-infected mice." in: PLoS neglected tropical diseases, Vol. 16, Issue 5, pp. e0010435, (2022) (PubMed).

Details zu CCL3

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Andere Bezeichnung: CCL3/MIP1 alpha

Hintergrund:

Background: Chemokine (C-C motif) ligand 3 (CCL3), so known as Macrophage inflammatory protein-1alpha (MIP-1 alpha), is a protein that in humans is encoded by the CCL3 gene. It is a cytokine which belongs to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. This gene is mapped to 17q12. CCL3 plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. It has been found that the activation of CCR5 by CCL3 ly and independently activates a G-protein signaling pathway through GNAI2 and a tyrosine phosphorylation signaling pathway through JAK2.

Gene Full Name: C-C motif chemokine ligand 3

Gen-ID: 20302

NCBI Accession: NP_035467

UniProt: P10855

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagie