FLT3LG ELISA Kit

Produktnummer ABIN6719986

Hersteller Produkt- Nr.: koa0172

Human FLT3LG ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human FLT3LG.

Kurzübersicht für FLT3LG ELISA Kit (ABIN6719986)

Target: FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6 pg/mL - 1000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum

Produktdetails FLT3LG ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Hersteller Produkt- Nr.: koa0172

Hersteller: Rockland

Verwendungszweck: Human Flt-3ligand Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human Flt-3ligand. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human Flt-3ligand standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each human Flt-3ligand standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: sf21, Immunogen sequence: T27-P185

Endotoxin level: Low endotoxin

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu FLT3LG

Target: FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

Andere Bezeichnung: Flt-3ligand

Hintergrund:

Synonyms: Flt 3L, Flt3 L, FLT3 LG, Flt3L, FLT3LG, Fms related tyrosine kinase 3 ligand, SL cytokine

Background: Fms-related tyrosine kinase 3 ligand(FLT3LG), also known as Flt-3ligand or FL, is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 19q13.3. Flt-3ligand is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor(SCF) and colony stimulating factor 1(CSF-1). In synergy with other growth factors, Flt-3ligand stimulates the proliferation and differentiation of various blood cell progenitors. Besides that, Flt-3ligand can control the development of Dendritic cells(DCs) and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. Flt-3ligand also can enhance the response of stem and primitive progenitor cells to other growth factors to generate all myeloid lineages except erythroid cells.

Gen-ID: 2323

NCBI Accession: NP_001191431

UniProt: P49771

Pathways: RTK Signalweg