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FASL ELISA Kit

Dieses Human FASL ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human FASL in Cell Culture Supernatant, Plasma (EDTA - heparin - citrate) und Serum zu quantifizieren.
Rockland
Produktnummer ABIN6719985
Hersteller Produkt- Nr.: koa0165

Kurzübersicht für FASL ELISA Kit (ABIN6719985)

Target

Alle FASL ELISA Kits anzeigen
FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reaktivität

  • 10
  • 5
  • 5
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
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  • 1
Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

15.6 pg/mL - 1000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
  • Untere Nachweisgrenze

    15.6 pg/mL

    Hersteller Produkt- Nr.

    koa0165

    Hersteller

    Rockland

    Verwendungszweck

    Human FASL Sandwich ELISA Kit for Quantitative Detection

    Marke

    AccuSignal™

    Analytische Methode

    Quantitative

    Spezifität

    Production: Natural and recombinant human FASL. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    < 2 pg/mL

    Bestandteile

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • Applikationshinweise

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human FASL standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each human FASL standard solution and each sample be measured in duplicate.

    Kommentare

    Standard: Expression system for standard: CHO, Immunogen sequence: P134-L281

    Probenmenge

    100 μL

    Plattentyp

    Pre-coated

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    Haltbarkeit

    12 months
  • Target Alle FASL ELISA Kits anzeigen

    FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

    Andere Bezeichnung

    FASL

    Hintergrund

    Synonyms: Apoptosis (APO 1) antigen ligand 1, Apoptosis antigen ligand, APT1LG1, APTL, CD178, CD95 L, CD95 ligand, CD95L, CD95L protein, Fas antigen ligand, FASL, FASLG, TNF superfamily member 6, TNFSF6, Tumor necrosis factor (ligand) superfamily member 6, Tumor necrosis factor ligand superfamily member 6

    Background: Fas ligand(FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor(TNF) family. Its binding with its receptor induces apoptosis. The human FASL gene consists of approximately 8 kb and is split into 4 exons. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Fas ligand or FasL is a homotrimeric type II transmembrane protein. It signals through trimerization of FasR, which spans the membrane of the "target" cell. This trimerization usually leads to apoptosis, or cell death. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7.

    Gen-ID

    14103

    NCBI Accession

    NP_001192172

    UniProt

    Q99PH8

    Pathways

    Apoptose, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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