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IL-13 ELISA Kit

Ratte IL-13 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Ratte IL-13.
Rockland
Produktnummer ABIN6719968
Hersteller Produkt- Nr.: koa0541

Kurzübersicht für IL-13 ELISA Kit (ABIN6719968)

Target

Alle IL-13 (IL13) ELISA Kits anzeigen
IL-13 (IL13) (Interleukin 13 (IL13))

Reaktivität

  • 11
  • 6
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Ratte

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

15.6 pg/mL - 1000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • Untere Nachweisgrenze

    15.6 pg/mL

    Hersteller Produkt- Nr.

    koa0541

    Hersteller

    Rockland

    Verwendungszweck

    Rat IL-13 Sandwich ELISA Kit for Quantitative Detection

    Marke

    AccuSignal™

    Analytische Methode

    Quantitative

    Spezifität

    Production: Natural and recombinant rat IL-13. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    < 1 pg/mL

    Bestandteile

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • Applikationshinweise

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL rat IL-13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each rat IL-13 standard solution and each sample be measured in duplicate.

    Kommentare

    Standard: Expression system for standard: E.coli, Immunogen sequence: T19-H131

    Probenmenge

    100 μL

    Plattentyp

    Pre-coated

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    Haltbarkeit

    12 months
  • Target Alle IL-13 (IL13) ELISA Kits anzeigen

    IL-13 (IL13) (Interleukin 13 (IL13))

    Andere Bezeichnung

    IL-13

    Hintergrund

    Synonyms: Allergic rhinitis, ALRH, BHR 1, BHR1, Bronchial hyperresponsiveness 1 (bronchial asthma), IL 13, IL-13, Il13, IL13_HUMAN, Interleukin 13, Interleukin-13, Interleukin13, MGC116786, MGC116788, MGC116789, NC30, P600

    Background: Interleukin 13(IL-13) is a protein that in humans is encoded by the IL13 gene. IL-13 is cytokine secreted by many cell types, but especially T helper type 2(Th2) cells, that is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor(IL-4Ralpha) and at least one of two known IL-13-specific binding chains. Most of the biological effects of IL-13, like those of IL-4, are linked to a single transcription factor, signal transducer and activator of transcription 6(STAT6). The IL13 gene is clustered with IL3, IL5, IL4, and CSF2 on 5q. Physical maps of the 5q23-q31 region show the following order: cen--IL3--CSF2--IL13--IL4--IL5--tel, with IL13 particularly close to IL4.

    Gen-ID

    116553

    NCBI Accession

    NP_446280

    UniProt

    P42203

    Pathways

    JAK-STAT Signalweg, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport
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