MMP2 ELISA Kit

Produktnummer ABIN6719913

Hersteller Produkt- Nr.: koa0368

Dieses Ratte MMP2 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Ratte MMP2 zu quantifizieren.

Kurzübersicht für MMP2 ELISA Kit (ABIN6719913)

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 625 pg/mL - 40000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (heparin), Serum

Produktdetails MMP2 ELISA Kit

Untere Nachweisgrenze: 625 pg/mL

Hersteller Produkt- Nr.: koa0368

Hersteller: Rockland

Verwendungszweck: Rat MMP-2 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant rat total MMP-2. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 40000pg/mL, 20,000pg/mL,10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL rat MMP-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma (heparin) to each empty well. It is recommended that each rat MMP-2 standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: NSO, Immunogen sequence: A30-C662

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu MMP2

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Andere Bezeichnung: MMP-2

Hintergrund:

Synonyms: 72 kDa gelatinase, 72 kDa type IV collagenase, CLG 4, CLG 4A, CLG4, CLG4A, Collagenase type IV A, EC 3.4.24.24, Gelatinase A, Gelatinase alpha, Gelatinase neutrophil, Matrix metallopeptidase 2 (gelatinase A, 72 kDa gelatinase, 72 kDa type IV collagenase), Matrix metalloproteinase 2, Matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IV collagenase), Matrix metalloproteinase II, Matrix metalloproteinase-2, MMP 2, MMP II, MMP-2, MMP2, MMP2_HUMAN, MONA, Neutrophil gelatinase, PEX, TBE 1, TBE-1

Background: Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2(MMP2). It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown.1 Matrix metalloproteinases(MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide(cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability.2 The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization.3 The standard product used in this kit is recombinant rat MMP-2, consisting of 662 amino acids with the molecular mass of 72KDa. The detected MMP-2 includes zymogen and active enzyme.

Gen-ID: 81686

NCBI Accession: NP_112316

UniProt: P33436

Pathways: Activation of Innate immune Response