Kallikrein 11 ELISA Kit

Produktnummer ABIN6719900

Hersteller Produkt- Nr.: koa0669

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human Kallikrein 11.

Kurzübersicht für Kallikrein 11 ELISA Kit (ABIN6719900)

Target: Kallikrein 11 (KLK11)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Applikation: ELISA

Proben: Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate

Produktdetails Kallikrein 11 ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Hersteller Produkt- Nr.: koa0669

Hersteller: Rockland

Verwendungszweck: Human KLK11 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human KLK11. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human KLK11 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human KLK11 standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: NSO, Immunogen sequence: E19-N250

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Kallikrein 11

Target: Kallikrein 11 (KLK11)

Andere Bezeichnung: KLK11

Hintergrund:

Synonyms: Hippostasin, hK11, Kallikrein 11, Kallikrein 11 isoform 1 preproprotein, Kallikrein 11 isoform 2 precursor, Kallikrein 11 precursor, Kallikrein related peptidase 11, Kallikrein-11 inactive chain 2, KLK 11, Klk11, KLK11_HUMAN, MGC33060, Protease serine 20 trypsin like, Protease serine trypsin like, PRSS20, Serine protease 20, TLSP, Trypsin like protease, Trypsin-like protease

Background: Kallikrein-11 is a protein that in humans is encoded by the KLK11 gene. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in two transcript variants encoding two different isoforms which are differentially expressed.

Gen-ID: 11012

NCBI Accession: NP_001129504

UniProt: Q9UBX7

Pathways: Komplementsystem