IL-4 ELISA Kit

Produktnummer ABIN6719863

Hersteller Produkt- Nr.: koa0221

Maus IL-4 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus IL-4.

Kurzübersicht für IL-4 ELISA Kit (ABIN6719863)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.8 pg/mL - 500 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum

Produktdetails IL-4 ELISA Kit

Untere Nachweisgrenze: 7.8 pg/mL

Hersteller Produkt- Nr.: koa0221

Hersteller: Rockland

Verwendungszweck: Mouse IL-4 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant mouse IL-4. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 1 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL mouse IL-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. We recommend that each mouse IL-4 standard solution and each sample is measured in duplicate.

Kommentare:

Standard: Expression system for standard: E.coli, Immunogen sequence: H21-S140

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu IL-4

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Andere Bezeichnung: IL-4

Hintergrund:

Synonyms: B cell growth factor 1, B cell stimulatory factor 1, BCGF 1, BCGF1, Binetrakin, BSF 1, BSF1, IL 4, Interleukin 4, Lymphocyte stimulatory factor 1, MGC79402, Pitrakinra

Background: Interleukin-4(IL-4), also knowns as a B-cell stimulatory factor1(BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells. The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis.1 The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5.

Gen-ID: 16189

NCBI Accession: NP_067258

UniProt: P07750

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation