Neurotrophin 4 ELISA Kit

Produktnummer ABIN6719849

Hersteller Produkt- Nr.: koa0282

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human Neurotrophin 4.

Kurzübersicht für Neurotrophin 4 ELISA Kit (ABIN6719849)

Target: Neurotrophin 4 (NTF4)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2 pg/mL - 2000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum

Produktdetails Neurotrophin 4 ELISA Kit

Untere Nachweisgrenze: 31.2 pg/mL

Hersteller Produkt- Nr.: koa0282

Hersteller: Rockland

Verwendungszweck: Human NT-4 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human NT-4. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human NT-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. It is recommended that each human NT-4 standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: sf21, Immunogen sequence: G81-A210

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Neurotrophin 4

Target: Neurotrophin 4 (NTF4)

Andere Bezeichnung: NT-4

Hintergrund:

Synonyms: GLC10, GLC1O, Neurotrophic factor 4, Neurotrophic factor 5, Neurotrophin 5, Neurotrophin 5 (neurotrophin 4/5), Neurotrophin-4, Neurotrophin-5, Neutrophic factor 4, Neutrophic factor 5, NT 4, NT 4/5, NT 5, NT-4, NT-5,NT4/5, NT4P, NT5, NTF4, NTF4_HUMAN, NTF5

Background: Neurotrophin-4(NT-4), also known as neurotrophin-5(NT-5), is a protein that in humans is encoded by the NTF4 gene. Human NT-4 as well as a human NT-4 pseudogene colocalize to chromosome 19 band q13.3. NT-4 is a member of a family of neurotrophic factors, the neurotrophins, that control survival and differentiation of vertebrate neurons(2-4). NT-4 is a neurotrophic factor that signals predominantly through the TrkB receptor tyrosine kinase. NT4 plays a physiological role essential for hippocampus- and amygdala-dependent long-term memory and hippocampal long-lasting LTP and that NT4 may be useful in the therapy of acquired disorders of learning and memory.

Gen-ID: 4909

NCBI Accession: NP_006170

UniProt: P34130

Pathways: RTK Signalweg