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Angiotensin I Converting Enzyme 1 ELISA Kit

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human Angiotensin I Converting Enzyme 1 in Cell Culture Supernatant, Plasma (heparin), Saliva und Serum.
Rockland
Produktnummer ABIN6719828
Hersteller Produkt- Nr.: koa0337

Kurzübersicht für Angiotensin I Converting Enzyme 1 ELISA Kit (ABIN6719828)

Target

Alle Angiotensin I Converting Enzyme 1 (ACE) ELISA Kits anzeigen
Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

Reaktivität

  • 6
  • 5
  • 5
  • 3
  • 2
  • 2
  • 2
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  • 1
  • 1
Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

156 pg/mL - 10000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Plasma (heparin), Saliva, Serum
  • Untere Nachweisgrenze

    156 pg/mL

    Hersteller Produkt- Nr.

    koa0337

    Hersteller

    Rockland

    Verwendungszweck

    Human ACE Sandwich ELISA Kit for Quantitative Detection

    Marke

    AccuSignal™

    Analytische Methode

    Quantitative

    Spezifität

    Production: Natural and recombinant human ACE. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    < 5 pg/mL

    Bestandteile

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • Applikationshinweise

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL human ACE standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants, serum, plasma(heparin) or saliva to each empty well. We recommend that each human ACE standard solution and each sample is measured in duplicate.

    Kommentare

    Standard: Expression system for standard: sf2NSO, Immunogen sequence: L30-L1261

    Probenmenge

    100 μL

    Plattentyp

    Pre-coated

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    Haltbarkeit

    12 months
  • Target Alle Angiotensin I Converting Enzyme 1 (ACE) ELISA Kits anzeigen

    Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

    Andere Bezeichnung

    ACE

    Hintergrund

    Synonyms: ACE, ACE_HUMAN, ACE1, Angiotensin converting enzyme somatic isoform, Angiotensin I converting enzyme (peptidyl dipeptidase A) 1, Angiotensin-converting enzyme, Carboxycathepsin, CD143, CD143 antigen, DCP, DCP1, Dipeptidyl carboxypeptidase 1, Dipeptidyl carboxypeptidase I, Kininase II, MVCD3, Peptidase P, soluble form, Testicular ECA

    Background: Angiotensin-converting enzyme(ACE) is a zinc-containing dipeptidyl carboxypeptidase widely distributed in mammalian tissues and is thought to play a critical role in blood pressure regulation. The predicted protein is identical, from residue 37 to its C terminus, to the second half or C-terminal domain of the endothelial ACE sequence. The protein sequence inferred consists of a 732-residue preprotein including a 31-residue signal peptide. The mature polypeptide has a molecular weight of 80,073. Although ACE has been studied primarily in the context of its role in blood pressure regulation, this widely distributed enzyme has many other physiological functions. The ACE gene encodes two isozymes. The somatic isozyme is expressed in many tissues, including vascular endothelial cells, renal epithelial cells, and testicular Leydig cells, whereas the testicular or germinal angiotensin-converting enzyme is expressed only in sperm. The standard product used in this kit is recombinant human ACE, consisting of 30-1261 amino acids with the molecular mass of 120KDa.

    Gen-ID

    1636

    NCBI Accession

    NP_000780

    UniProt

    B4DKH4

    Pathways

    ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration
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