CD32-B/C ELISA Kit

Produktnummer ABIN6719821

Hersteller Produkt- Nr.: koa0785

Human CD32-B/C ELISA Kit, Colorimetric Assay für die Quantifizierung von Human CD32-B/C.

Kurzübersicht für CD32-B/C ELISA Kit (ABIN6719821)

Target: CD32-B/C

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 46.9 pg/mL - 3000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Produktdetails

Untere Nachweisgrenze: 46.9 pg/mL

Hersteller Produkt- Nr.: koa0785

Hersteller: Rockland

Verwendungszweck: Human CD32 - FCGR2b - c Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human CD32. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL, 419.pg/mL, 187.5pg/mL, 93.8pg/mL, 46.9pg/mL human CD32 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human CD32 standard solution and each sample is measured in duplicate.

Kommentare:

Standard: Expression system for standard: NSO, Immunogen sequence: A46-P217

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Antigendetails

Target: CD32-B/C

Andere Bezeichnung: CD32/FCGR2b/c

Hintergrund:

Synonyms: CD32 antigen, CD32A, CD32B, CD32C, CDw32, Fc fragment of IgG low affinity IIa receptor, Fc fragment of IgG low affinity IIb receptor, Fc fragment of IgG low affinity IIc receptor, Fc-gamma RII-a, Fc-gamma RII-b, Fc-gamma RII-c, Fc-gamma-RIIa, FC-gamma-RIIB, FCG2, FCG2A_HUMAN, FcgammaRIIC, FcGR, FCGR2, FCGR2A, FCGR2C, Fcgr3, Fcgr3a, Fcr-2, Fcr-3, FcRII a, FCRII, FcRII b, FcRIIC, IGFR 2, IGFR2, IgG Fc receptor II a, IgG Fc receptor II a, Low affinity immunoglobulin gamma Fc region receptor II, Lymphocyte antigen 17

Background: CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.

Gen-ID: 9103

NCBI Accession: NP_963857

UniProt: P31995