CD32-B/C ELISA Kit
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- Target
- CD32-B/C
- Reaktivität
- Maus
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 31.2 pg/mL - 2000 pg/mL
- Untere Nachweisgrenze
- 31.2 pg/mL
- Applikation
- ELISA
- Verwendungszweck
- Sandwich ELISA for Quantitative Detection of Antigen
- Proben
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytische Methode
- Quantitative
- Produktmerkmale
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Synonyms: CD32 antigen, CD32A, CD32B, CD32C, CDw32, Fc fragment of IgG low affinity IIa receptor, Fc fragment of IgG low affinity IIb receptor, Fc fragment of IgG low affinity IIc receptor, Fc-gamma RII-a, Fc-gamma RII-b, Fc-gamma RII-c, Fc-gamma-RIIa, FC-gamma-RIIB, FCG2, FCG2A_HUMAN, FcgammaRIIC, FcGR, FCGR2, FCGR2A, FCGR2C, Fcgr3, Fcgr3a, Fcr-2, Fcr-3, FcRII a, FCRII, FcRII b, FcRIIC, IGFR 2, IGFR2, IgG Fc receptor II a, IgG Fc receptor II a, Low affinity immunoglobulin gamma Fc region receptor II, Lymphocyte antigen 17
Background: CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.
Gene Name: FCGR2B
Production: Natural and recombinant mouse CD32. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: NSO, Immunogen sequence: T40-R217
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CD32-B/C ELISA Kit
Reaktivität: Human Colorimetric Sandwich ELISA 85-5000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- Applikationshinweise
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse CD32 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CD32 standard solution and each sample be measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 31.2pg/mL-2000pg/mL
- Probenmenge
- 100 μL
- Plattentyp
- Pre-coated
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- RT,4 °C,-20 °C
- Informationen zur Lagerung
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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