MICB ELISA Kit

Produktnummer ABIN6719775

Produktdetails MICB ELISA Kit

Target: MICB (MHC Class I Polypeptide-Related Sequence B (MICB))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: MHC class I chain related protein B, MHC class I like molecule PERB11.2 IMX, MHC class I mic B antigen, MHC class I polypeptide related sequence B, MHC class I polypeptide-related sequence B, MIC B, MIC-B, MICB, MICB_HUMAN, PERB11.2, Stress inducible class I homolog

Background: MHC class I polypeptide-related sequence B, also called MICB is a protein that in humans is encoded by the MICB gene. The MICB gene is mapped to 6p21.33. This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer(NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules, however, it does not associate with beta-2-microglobulin or bind peptides. It seems to have no role in antigen presentation. This gene acts as a stress-induced self-antigen that is recognized by gamma delta T cells. It is the ligand for the KLRK1/NKG2D receptor and binds to KLRK1 leads to cell lysis.

Gene Name: MICB

Production: Natural and recombinant human MICB. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: A23-G298

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human MICB standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human MICB standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu MICB

Target: MICB (MHC Class I Polypeptide-Related Sequence B (MICB))

Andere Bezeichnung: MICB (MICB Produkte)

Synonyme: PERB11.2 ELISA Kit, MHC class I polypeptide-related sequence B ELISA Kit, MHC class I-related protein-like ELISA Kit, MICB ELISA Kit, LOC512825 ELISA Kit

Gen-ID: 4277

NCBI Accession: NP_001276089

UniProt: Q29980