Prostate Specific Antigen ELISA Kit

Produktnummer ABIN6719774

Hersteller Produkt- Nr.: koa0486

Produktdetails Prostate Specific Antigen ELISA Kit

Target: Prostate Specific Antigen (PSA)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 312 pg/mL - 20000 pg/mL

Untere Nachweisgrenze: 312 pg/mL

Applikation: ELISA

Hersteller Produkt- Nr.: koa0486

Hersteller: Rockland

Verwendungszweck: Human Kallikrein 3 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human Kallikrein 3. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human Kallikrein 3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Kallikrein 3 standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: NSO, Immunogen sequence: A18-P261

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Prostate Specific Antigen

Target: Prostate Specific Antigen (PSA)

Andere Bezeichnung: Kallikrein 3 (PSA Produkte)

Hintergrund:

Synonyms: APS, Gamma seminoprotein, Gamma-seminoprotein, hK3, Kallikrein 3, Kallikrein related peptidase 3, Kallikrein-3, KLK 3, KLK2A1, KLK3, Klk3, KLK3_HUMAN, P 30 antigen, P-30 antigen, P30 antigen, Prostate Specific Antigen, Prostate-specific antigen, Psa, Semenogelase, Seminin

Background: Prostate-specific antigen(PSA), also known as kallikrein III, seminin, semenogelase, gamma-seminoprotein and P-30 antigen) is a 34 kD glycoprotein manufactured almost exclusively by the prostate gland, PSA is produced for the ejaculate where it liquifies the semen in the seminal coagulum and allows sperm to swim freely.1It is also believed to be instrumental in dissolving the cervical mucous cap, allowing the entry of sperm.2It is a serine protease enzyme, the gene of which is located on the nineteenth chromosome(19q13).3

Gen-ID: 354

NCBI Accession: NP_001025218

UniProt: P07288

Pathways: Komplementsystem