PAI1 ELISA Kit

Produktnummer ABIN6719739

Hersteller Produkt- Nr.: koa0520

Dieses Human PAI1 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human PAI1 zu quantifizieren.

Kurzübersicht für PAI1 ELISA Kit (ABIN6719739)

Target: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum

Produktdetails PAI1 ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Hersteller Produkt- Nr.: koa0520

Hersteller: Rockland

Verwendungszweck: Human PAI-1 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human PAI-1. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human PAI-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, serum or plasma(heparin, EDTA, citrate ) to each empty well. It is recommended that each human PAI-1 standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: sf21, Immunogen sequence: S22-P402

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu PAI1

Target: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Andere Bezeichnung: PAI-1

Hintergrund:

Synonyms: Endothelial plasminogen activator inhibitor, PAI, PAI-1, PAI1, PAI1_HUMAN, PLANH1, Plasminogen activator inhibitor 1, Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1, Serpin E1, Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1, SERPINE1

Background: Plasminogen activator inhibitor-1 is the principal inhibitor of tissue plasminogen activator(tPA) and urokinase(uPA), the activators of plasminogen and hence fibrinolysis(the physiological breakdown of blood clots). It is a serine protease inhibitor(serpin) protein(SERPINE1). PAI-1 is mainly produced by the endothelium(cells lining blood vessels), but is also secreted by other tissue types, such as adipose tissue. The PAI-1 gene is located on the seventh chromosome(7q21.3-q22).1 Congenital deficiency of PAI-1 leads to a hemorrhagic diathesis(a tendency to hemorrhage). PAI-1 is present in increased levels in various disease states(such as a number of forms of cancer), as well as in obesity and the metabolic syndrome. It has been linked to the increased occurrence of thrombosis in patients with these conditions. Additionally, it has been shown to interact with ORM1.2 The standard product used in this kit is recombinant human PAI-1 with the molecular mass of 43KDa.

Gen-ID: 5054

NCBI Accession: NP_000593

UniProt: P05121

Pathways: p53 Signalweg, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagie, Smooth Muscle Cell Migration