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MICA ELISA Kit (MHC Class I Polypeptide-Related Sequence A) ELISA Kit

MICA Reaktivität: Human Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Lysate, Cell Culture Supernatant, Serum, Tissue Lysate
Produktnummer ABIN6719703
$552.00
Zzgl. Versandkosten $45.00
1 kit
Lieferung in 1 bis 2 Werktagen
  • Target Alle MICA ELISA Kits anzeigen
    MICA
    Reaktivität
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    62.5 pg/mL - 4000 pg/mL
    Untere Nachweisgrenze
    62.5 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich ELISA for Quantitative Detection of Antigen
    Proben
    Cell Culture Lysate, Cell Culture Supernatant, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Produktmerkmale

    Synonyms: FLJ60820, HLA class I antigen, MGC111087, MHC class I chain related gene A protein, MHC class I chain related protein A, MHC class I polypeptide related sequence A, MHC class I related protein, MIC A, PERB11.1, Stress inducible class I homolog

    Background: MHC class I polypeptide-related sequence A is a protein that in humans is encoded by the MICA gene.1 The MICA gene encodes a 383-amino acid polypeptide with a predicted mass of 43 kD.The MICA and MICB genes occur in a 200-kb region spanning the TNFA and TNFB cluster at 6p21.3.2 MICA and the closely related MICB were recognized by intestinal epithelial T cells expressing diverse V-delta-1 gamma/delta TCRs.3 The MICA protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is further distinguished by its unusual exon-intron organization and preferential expression in fibroblasts and epithelial cells. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by NK cells, NKT cells, and most of the subtypes of T cells. MICA and other members of this family may have been selected for specialized functions that are either ancient or derived from those of typical MHC class I genes, in analogy to some of the nonclassic mouse H-2 genes.

    Gene Name: MICA

    Production: Natural and recombinant human MICA. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: NSO, Immunogen sequence: E24-W307

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  • Applikationshinweise

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human MICA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue lysates or serum to each empty well. It is recommended that each human MICA standard solution and each sample be measured in duplicate.

    ELISA Dilution: 62.5pg/mL-4000pg/mL

    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    RT,4 °C,-20 °C
    Informationen zur Lagerung
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • Target Alle MICA ELISA Kits anzeigen
    MICA
    Andere Bezeichnung
    MICA (MICA Produkte)
    Synonyme
    MIC-A, PERB11.1, MHC class I polypeptide-related sequence A, MICA
    Gen-ID
    100507436
    NCBI Accession
    NP_000238
    UniProt
    Q29983
    Pathways
    Activation of Innate immune Response, Transition Metal Ion Homeostasis, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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