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MICA ELISA Kit

This anti-MICA antibody (ABIN6719703) is a antibody detecting MICA in ELISA. Suitable for Human.
Rockland
Produktnummer ABIN6719703
Hersteller Produkt- Nr.: koa0483

Kurzübersicht für MICA ELISA Kit (ABIN6719703)

Target

Alle MICA ELISA Kits anzeigen
MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Reaktivität

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Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

62.5 pg/mL - 4000 pg/mL

Applikation

ELISA

Proben

Cell Culture Lysate, Cell Culture Supernatant, Serum, Tissue Lysate
  • Untere Nachweisgrenze

    62.5 pg/mL

    Hersteller Produkt- Nr.

    koa0483

    Hersteller

    Rockland

    Verwendungszweck

    Human MICA Sandwich ELISA Kit for Quantitative Detection

    Marke

    AccuSignal™

    Analytische Methode

    Quantitative

    Spezifität

    Production: Natural and recombinant human MICA. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    < 10 pg/mL

    Bestandteile

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • Applikationshinweise

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human MICA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue lysates or serum to each empty well. It is recommended that each human MICA standard solution and each sample be measured in duplicate.

    Kommentare

    Standard: Expression system for standard: NSO, Immunogen sequence: E24-W307

    Probenmenge

    100 μL

    Plattentyp

    Pre-coated

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    Haltbarkeit

    12 months
  • Target Alle MICA ELISA Kits anzeigen

    MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

    Andere Bezeichnung

    MICA

    Hintergrund

    Synonyms: FLJ60820, HLA class I antigen, MGC111087, MHC class I chain related gene A protein, MHC class I chain related protein A, MHC class I polypeptide related sequence A, MHC class I related protein, MIC A, PERB11.1, Stress inducible class I homolog

    Background: MHC class I polypeptide-related sequence A is a protein that in humans is encoded by the MICA gene.1 The MICA gene encodes a 383-amino acid polypeptide with a predicted mass of 43 kD. The MICA and MICB genes occur in a 200-kb region spanning the TNFA and TNFB cluster at 6p21.3.2 MICA and the closely related MICB were recognized by intestinal epithelial T cells expressing diverse V-delta-1 gamma/delta TCRs.3 The MICA protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is further distinguished by its unusual exon-intron organization and preferential expression in fibroblasts and epithelial cells. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by NK cells, NKT cells, and most of the subtypes of T cells. MICA and other members of this family may have been selected for specialized functions that are either ancient or derived from those of typical MHC class I genes, in analogy to some of the nonclassic mouse H-2 genes.

    Gen-ID

    100507436

    NCBI Accession

    NP_000238

    UniProt

    Q29983

    Pathways

    Activation of Innate immune Response, Transition Metal Ion Homeostasis, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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