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Kallikrein 8 ELISA Kit

Human Kallikrein 8 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human Kallikrein 8 in Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum und Tissue Homogenate
Rockland
Produktnummer ABIN6719701
Hersteller Produkt- Nr.: koa0489

Kurzübersicht für Kallikrein 8 ELISA Kit (ABIN6719701)

Target

Alle Kallikrein 8 (KLK8) ELISA Kits anzeigen
Kallikrein 8 (KLK8)

Reaktivität

  • 6
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Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

156 pg/mL - 10000 pg/mL

Applikation

ELISA

Proben

Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate
  • Untere Nachweisgrenze

    156 pg/mL

    Hersteller Produkt- Nr.

    koa0489

    Hersteller

    Rockland

    Verwendungszweck

    Human KLK8 - Kallikrein-8 Sandwich ELISA Kit for Quantitative Detection

    Marke

    AccuSignal™

    Analytische Methode

    Quantitative

    Spezifität

    Production: Natural and recombinant human KLK8. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    < 10 pg/mL

    Bestandteile

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • Applikationshinweise

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human KLK8 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human KLK8 standard solution and each sample be measured in duplicate.

    Kommentare

    Standard: Expression system for standard: NSO, Immunogen sequence: Q29-G260

    Probenmenge

    100 μL

    Plattentyp

    Pre-coated

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    Haltbarkeit

    12 months
  • Target Alle Kallikrein 8 (KLK8) ELISA Kits anzeigen

    Kallikrein 8 (KLK8)

    Andere Bezeichnung

    KLK8/Kallikrein-8

    Hintergrund

    Synonyms: Brain serine protease 1, BSP1, hK8, HNP, HPN, Kallikrein 8 (neuropsin/ovasin), Kallikrein related peptidase 8, KLK8 protein type 1, KLK8 protein type 2, KLK8_HUMAN, Neuropsin, Neuropsin type 1, Neuropsin type 2, NP, NRPN, Ovasin, PRO322, Protease serine 19, PRSS 19, Serine protease 19, Serine protease kallikrein, Serine protease kallikrein/ovasin/neuropsin, Serine protease TADG 14, TADG14, Tumor associated differentially expressed gene 14, Tumor associated differentially expressed gene 14 protein

    Background: Kallikrein-8 is a protein that in humans is encoded by the KLK8 gene. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in four transcript variants encoding four different isoforms. The isoforms exhibit distinct patterns of expression that suggest roles in brain plasticity and ovarian cancer.

    Gen-ID

    11202

    NCBI Accession

    NP_001268360

    UniProt

    O60259

    Pathways

    Komplementsystem
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