BMP2 ELISA Kit

Produktnummer ABIN6719672

Hersteller Produkt- Nr.: koa0150

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human BMP2.

Kurzübersicht für BMP2 ELISA Kit (ABIN6719672)

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2 pg/mL - 2000 pg/mL

Applikation: ELISA

Proben: Bone, Cell Culture Supernatant, Serum

Produktdetails BMP2 ELISA Kit

Untere Nachweisgrenze: 31.2 pg/mL

Hersteller Produkt- Nr.: koa0150

Hersteller: Rockland

Verwendungszweck: Human BMP-2 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human BMP-2. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 2 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human BMP-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human bone tissue, cell culture supernates or serum to each empty well. It is recommended that each human BMP-2 standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard:CHO , Immunogen sequence: Q283-R396

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu BMP2

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Andere Bezeichnung: BMP-2

Hintergrund:

Synonyms: BDA2, BMP-2, BMP-2A, Bmp2, BMP2_HUMAN, BMP2A, Bone morphogenetic protein 2, Bone morphogenetic protein 2A

Background: Bone morphogenetic protein-2(BMP-2, BMP 2A) belongs to the transforming growth factor beta(TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice.1 BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase(MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis.2 Bone morphogenic proteins(BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.3 The standard product used in this kit is recombinant human BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.

Gen-ID: 650

NCBI Accession: NP_001191

UniProt: C8C060

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation