Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

BMP2 ELISA Kit

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human BMP2.
Rockland
Produktnummer ABIN6719672
Hersteller Produkt- Nr.: koa0150

Kurzübersicht für BMP2 ELISA Kit (ABIN6719672)

Target

Alle BMP2 ELISA Kits anzeigen
BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Reaktivität

  • 6
  • 6
  • 4
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

31.2 pg/mL - 2000 pg/mL

Applikation

ELISA

Proben

Bone, Cell Culture Supernatant, Serum
  • Untere Nachweisgrenze

    31.2 pg/mL

    Hersteller Produkt- Nr.

    koa0150

    Hersteller

    Rockland

    Verwendungszweck

    Human BMP-2 Sandwich ELISA Kit for Quantitative Detection

    Marke

    AccuSignal™

    Analytische Methode

    Quantitative

    Spezifität

    Production: Natural and recombinant human BMP-2. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    < 2 pg/mL

    Bestandteile

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • Applikationshinweise

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human BMP-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human bone tissue, cell culture supernates or serum to each empty well. It is recommended that each human BMP-2 standard solution and each sample be measured in duplicate.

    Kommentare

    Standard: Expression system for standard:CHO , Immunogen sequence: Q283-R396

    Probenmenge

    100 μL

    Plattentyp

    Pre-coated

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    Haltbarkeit

    12 months
  • Target Alle BMP2 ELISA Kits anzeigen

    BMP2 (Bone Morphogenetic Protein 2 (BMP2))

    Andere Bezeichnung

    BMP-2

    Hintergrund

    Synonyms: BDA2, BMP-2, BMP-2A, Bmp2, BMP2_HUMAN, BMP2A, Bone morphogenetic protein 2, Bone morphogenetic protein 2A

    Background: Bone morphogenetic protein-2(BMP-2, BMP 2A) belongs to the transforming growth factor beta(TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice.1 BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase(MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis.2 Bone morphogenic proteins(BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.3 The standard product used in this kit is recombinant human BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.

    Gen-ID

    650

    NCBI Accession

    NP_001191

    UniProt

    C8C060

    Pathways

    Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation
Sie sind hier:
Chat with us!